Taccalonolides E and A: Plant-derived steroids with microtubule-stabilizing activity

• Published in: Cancer research (Chicago, Ill.) Vol. 63; no. 12; pp. 3211 - 3220
• Main Authors: TINLEY, Tina L, RANDALL-HLUBEK, Deborah A, LEAL, Rachel M, JACKSON, Evelyn M, CESSAC, James W, QUADA, James C, HEMSCHEIDT, Thomas K, MOOBERRY, Susan L
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.06.2003
• Subjects: Animals; Antineoplastic agents; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis - drug effects; Biological and medical sciences; Biopolymers; Breast Neoplasms - pathology; Cell Nucleus - drug effects; Cells, Cultured - drug effects; Cells, Cultured - ultrastructure; Centrosome - drug effects; Chemotherapy; Drug Resistance, Neoplasm; Enzyme Activation - drug effects; Female; General pharmacology; HeLa Cells - drug effects; HeLa Cells - ultrastructure; Humans; Interphase - drug effects; MAP Kinase Signaling System - drug effects; Medical sciences; Microtubule Proteins - metabolism; Microtubules - drug effects; Mitosis - drug effects; Molecular Structure; Muscle, Smooth - cytology; Ovarian Neoplasms - pathology; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phosphorylation - drug effects; Protein Processing, Post-Translational - drug effects; Proto-Oncogene Proteins c-bcl-2 - metabolism; Rats; Rhizome - chemistry; Spindle Apparatus - drug effects; Steroids - isolation & purification; Steroids - pharmacology; Tumor Cells, Cultured - drug effects; Tumor Cells, Cultured - ultrastructure; Antineoplastic agent; Human; Steroid; Monocotyledones; Root; Extract; In vitro; Biological activity; Medicinal plant; Tubulin; Animal; Angiospermae; Cell cycle; Established cell line; Plant origin; Cytoskeleton; Spermatophyta; Microtubule; Mechanism of action; Antimitotic; Taccaceae; Tumor cell

During the course of a mechanism-based screening program designed to identify new microtubule-disrupting agents from natural products, we identified a crude extract from Tacca chantrieri that initiated Taxol-like microtubule bundling. Bioassay-directed purification of the extract yielded the highly...