New Methodological Approach to Induce a Differentiation Phenotype in Caco-2 Cells Prior to Post-confluence Stage

Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of diffe...

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Published inAnticancer research Vol. 27; no. 6B; pp. 3919 - 3925
Main Authors FERRARETTO, Anita, GRAVAGHI, Claudia, DONETTI, Elena, COSENTINO, Stefania, DONIDA, Bianca M, BEDONI, Marzia, LOMBARDI, Giovanni, FIORILLI, Amelia, TETTAMANTI, Guido
Format Journal Article
LanguageEnglish
Published Attiki International Institute of Anticancer Research 01.11.2007
Subjects
Biological and medical sciences
Caco-2 Cells - enzymology
Caco-2 Cells - pathology
Caco-2 Cells - ultrastructure
Cell Count
Cell Culture Techniques - methods
Cell Differentiation - physiology
Cell Growth Processes - physiology
Humans
Medical sciences
Phenotype
Tumors
Cell proliferation
Phenotype
Clinical stage
Cancerology
Caco-2 cells
differentiation
intestine
proliferation
Digestive system
Confluence
Gut
Cell differentiation
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Summary:Background: Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. Materials and Methods: Ultrastructural analysis, cell proliferation activity and biochemical markers of differentiation were evaluated at different passages. Results: Subcultures of Caco-2 cells at pre-confluence, exhibiting progressive acquisition of a more benign differentiation phenotype, were generated. Early passages of Caco-2 cells showed a well-developed brush border and incomplete junctional apparatus; subsequent subcultures yielded cell populations with well-developed junctions similar to those of small intestinal cells. Conclusion: These culture conditions represent a new versatile model not only to progressively induce the differentiation program in Caco-2 cells at pre-confluence without changes of culture media, but also to explore mechanistic modes of drug transport and tumor development.
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ISSN:0250-7005
1791-7530