Light-induced photoactivation of hypericin affects the energy metabolism of human glioma cells by inhibiting hexokinase bound to mitochondria

• Published in: Cancer research (Chicago, Ill.) Vol. 58; no. 24; pp. 5777 - 5786
• Main Authors: MICCOLI, L, BEURDELEY-THOMAS, A, DE PINIEUX, G, SUREAU, F, OUDARD, S, DUTRILLAUX, B, POUPON, M.-F
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.12.1998
• Subjects: Apoptosis - drug effects; Biological and medical sciences; Energy Metabolism; Enzyme Inhibitors - pharmacology; Glioma - drug therapy; Glioma - metabolism; Hexokinase - antagonists & inhibitors; Hexokinase - metabolism; Humans; Hydrogen-Ion Concentration; Light; Medical sciences; Mitochondria - drug effects; Mitochondria - metabolism; Perylene - analogs & derivatives; Perylene - metabolism; Perylene - pharmacology; Photoradiation therapy and photosensitizing agent; Radiation-Sensitizing Agents - pharmacology; Thymidine - metabolism; Treatment with physical agents; Treatment. General aspects; Tumor Cells, Cultured; Tumors; Oxidative stress; Energy metabolism; Gene product; Cytotoxicity; Malignant glioma; Mitochondria; Ligases; Established cell line; pH; Mechanism of action; Tumor cell; Glutathione; Glutamate-cysteine ligase; Human; Nervous system diseases; Carbon-nitrogen ligases; Enzyme; Transferases; Enzyme inhibitor; Malignant tumor; Gene expression; In vitro; Hexokinase; Central nervous system disease; Photosensitizer; Apoptosis

Glucose-dependent energy required for glioma metabolism depends on hexokinase, which is mainly bound to mitochondria. A decrease in intracellular pH leads to a release of hexokinase-binding, which in turn decreases glucose phosphorylation, ATP content, and cell proliferation. Thus, intracellular pH...