Expression and Prognostic Value of Wilms’ Tumor 1 and Early Growth Response 1 Proteins in Nephroblastoma
Wilms’ tumor is one of the most common solid tumors of children. The protein product of the tumor-suppressor gene, Wilms’ tumor 1 (WT-1), binds to the same DNA sequences as the protein product of the early growth response 1 (EGR-1) gene. There is experimental evidence that EGR-1 is involved in contr...
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Published in | Clinical cancer research Vol. 6; no. 11; pp. 4265 - 4271 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Philadelphia, PA
American Association for Cancer Research
01.11.2000
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Subjects | |
Online Access | Get full text |
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Summary: | Wilms’ tumor is one
of the most common solid tumors of children. The protein product of the
tumor-suppressor gene, Wilms’ tumor 1 (WT-1), binds to the same DNA
sequences as the protein product of the early growth response 1 (EGR-1)
gene. There is experimental evidence that EGR-1 is involved in
controlling cell growth. The expression of both genes in Wilms’ tumor
was studied by others, mainly at the mRNA level. The present study
evaluates the prognostic value of WT-1 and EGR-1 in 61 Wilms’ tumors
of chemotherapeutically treated patients at the protein level, using an
immunohistochemical approach. WT-1 was expressed in normal kidney
tissues and in the blastemal and epithelial component of Wilms’ tumor,
whereas stromal tissue was negative. EGR-1 was expressed in normal
kidney tissues and in the three main cell types of Wilms’ tumor. In 59
and 56% of Wilms’ tumor, the blastemal cells stained for WT-1 and
EGR-1, respectively. The blastemal expression of WT-1 and EGR-1 and the
epithelial expression of WT-1 were statistically significantly
correlated with clinical stage. WT-1 immunoreactivity correlated with
EGR-1 expression. Univariate analysis showed that blastemal WT-1 and
EGR-1 expression were indicative for clinical progression and
tumor-specific survival, whereas epithelial staining was of no
prognostic value. Multivariate analysis showed that blastemal WT-1
expression is an independent prognostic marker for clinical progression
other than stage. We conclude that a relationship exists between WT-1
and EGR-1 expression in clinical nephroblastomas. Blastemal WT-1 and
EGR-1 expression is related to prognosis. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1078-0432 1557-3265 |