Cryopreservation of Equine Sperm: Optimal Cooling Rates in the Presence and Absence of Cryoprotective Agents Determined Using Differential Scanning Calorimetry

• Published in: Biology of reproduction Vol. 66; no. 1; pp. 222 - 231
• Main Authors: DEVIREDDY, R. V, SWANLUND, D. J, OLIN, T, VINCENTE, W, TROEDSSON, M. H. T, BISCHOF, J. C, ROBERTS, K. P
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction 01.01.2002
• Subjects: Algorithms; Animals; Biological and medical sciences; Calorimetry, Differential Scanning; Cell Membrane - physiology; Cell Membrane - ultrastructure; Cell Survival - physiology; Computer Simulation; Cryopreservation; Cryoprotective Agents - pharmacology; Freezing; Horses - physiology; In Vitro Techniques; Male; Semen Preservation; Spermatozoa - drug effects; Spermatozoa - physiology; Temperature; Water - metabolism

Optimization of equine sperm cryopreservation protocols requires an understanding of the water permeability characteristics and volumetric shrinkage response during freezing. A cell-shape-independent differential scanning calorimeter (DSC) technique was used to measure the volumetric shrinkage durin...