Significant Differences Between Mouse and Human Trophinins Are Revealed by Their Expression Patterns and Targeted Disruption of Mouse Trophinin Gene
Trophinin has been identified as a membrane protein mediating apical cell adhesion between two human cell lines: trophoblastic HT-H cells, and endometrial epithelial SNG-M cells. Expression patterns of trophinin in humans suggested its involvement in embryo implantation and early placental developme...
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Published in | Biology of reproduction Vol. 66; no. 2; pp. 313 - 321 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Madison, WI
Society for the Study of Reproduction
01.02.2002
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Subjects | |
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Abstract | Trophinin has been identified as a membrane protein mediating apical cell adhesion between two human cell lines: trophoblastic
HT-H cells, and endometrial epithelial SNG-M cells. Expression patterns of trophinin in humans suggested its involvement in
embryo implantation and early placental development. The mouse trophinin gene maps to the distal part of the X chromosome
and corresponds to human chromosome Xp11.21â22, the locus where the human trophinin gene maps. Western blot analysis indicates
that the molecular weight of mouse trophinin is 110 kDa, which is consistent with the calculated value of 107 kDa. Positive
signals for trophinin proteins were detected in preimplantation mouse embryos at the morula and blastocyst stages. Implanting
blastocysts do not show detectable levels of trophinin protein, demonstrating that trophinin is not involved in blastocyst
adhesion to the uterus in the mouse. Mouse embryo strongly expressed trophinin in the epiblast 1 day after implantation. Trophinin
protein was not found in the mouse uteri and placenta after 5.5 days postcoitus (dpc). Targeted disruption of the trophinin
gene in the mouse showed a partial embryonic lethality in a 129/SvJ background, but the cause of this lethality remains undetermined.
The present study indicates significant differences between mouse and human trophinins in their expression patterns, and it
suggests that trophinin is not involved in embryo implantation and placental development in the mouse. |
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AbstractList | Trophinin has been identified as a membrane protein mediating apical cell adhesion between two human cell lines: trophoblastic HT-H cells, and endometrial epithelial SNG-M cells. Expression patterns of trophinin in humans suggested its involvement in embryo implantation and early placental development. The mouse trophinin gene maps to the distal part of the X chromosome and corresponds to human chromosome Xp11.21-22, the locus where the human trophinin gene maps. Western blot analysis indicates that the molecular weight of mouse trophinin is 110 kDa, which is consistent with the calculated value of 107 kDa. Positive signals for trophinin proteins were detected in preimplantation mouse embryos at the morula and blastocyst stages. Implanting blastocysts do not show detectable levels of trophinin protein, demonstrating that trophinin is not involved in blastocyst adhesion to the uterus in the mouse. Mouse embryo strongly expressed trophinin in the epiblast 1 day after implantation. Trophinin protein was not found in the mouse uteri and placenta after 5.5 days postcoitus (dpc). Targeted disruption of the trophinin gene in the mouse showed a partial embryonic lethality in a 129/SvJ background, but the cause of this lethality remains undetermined. The present study indicates significant differences between mouse and human trophinins in their expression patterns, and it suggests that trophinin is not involved in embryo implantation and placental development in the mouse. Trophinin has been identified as a membrane protein mediating apical cell adhesion between two human cell lines: trophoblastic HT-H cells, and endometrial epithelial SNG-M cells. Expression patterns of trophinin in humans suggested its involvement in embryo implantation and early placental development. The mouse trophinin gene maps to the distal part of the X chromosome and corresponds to human chromosome Xp11.21â22, the locus where the human trophinin gene maps. Western blot analysis indicates that the molecular weight of mouse trophinin is 110 kDa, which is consistent with the calculated value of 107 kDa. Positive signals for trophinin proteins were detected in preimplantation mouse embryos at the morula and blastocyst stages. Implanting blastocysts do not show detectable levels of trophinin protein, demonstrating that trophinin is not involved in blastocyst adhesion to the uterus in the mouse. Mouse embryo strongly expressed trophinin in the epiblast 1 day after implantation. Trophinin protein was not found in the mouse uteri and placenta after 5.5 days postcoitus (dpc). Targeted disruption of the trophinin gene in the mouse showed a partial embryonic lethality in a 129/SvJ background, but the cause of this lethality remains undetermined. The present study indicates significant differences between mouse and human trophinins in their expression patterns, and it suggests that trophinin is not involved in embryo implantation and placental development in the mouse. |
Author | Michiko N. Fukuda Bibhash C. Paria Jun Nakayama Daita Nadano Debra J. Gilbert Nancy A. Jenkins Sakura Saburi Kazuhiro Sugihara Neal G. Copeland |
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Snippet | Trophinin has been identified as a membrane protein mediating apical cell adhesion between two human cell lines: trophoblastic
HT-H cells, and endometrial... Trophinin has been identified as a membrane protein mediating apical cell adhesion between two human cell lines: trophoblastic HT-H cells, and endometrial... |
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SubjectTerms | Amino Acid Sequence Animals Biological and medical sciences Blastocyst - physiology Blotting, Southern Blotting, Western Cell Adhesion Molecules - biosynthesis Cell Adhesion Molecules - genetics Cell Adhesion Molecules - physiology Chromosome Mapping Cloning, Molecular Female Gene Expression Regulation, Developmental Genome Genotype Humans Immunohistochemistry Inbreeding Mice Mice, Inbred C57BL Molecular Sequence Data Reverse Transcriptase Polymerase Chain Reaction Species Specificity X Chromosome - genetics |
Title | Significant Differences Between Mouse and Human Trophinins Are Revealed by Their Expression Patterns and Targeted Disruption of Mouse Trophinin Gene |
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