Thromboplastin-thrombomodulin-mediated time: a new global test sensitive to protein S deficiency and increased levels of factors II, V, VII and X

• Published in: Haematologica (Roma) Vol. 87; no. 4; pp. 415 - 419
• Main Authors: Borrell, M, Llobet, D, Ortin, R, Felices, R, Vallve, C, Mateo, J, Souto, J, Fontcuberta, J
• Format: Journal Article
• Language: English
• Published: Pavia Haematologica 01.04.2002
• Subjects: Biological and medical sciences; Blood Coagulation - drug effects; Blood Coagulation Factors - pharmacology; Blood Coagulation Tests - methods; Blood Coagulation Tests - standards; Case-Control Studies; Female; Hematologic and hematopoietic diseases; Humans; Male; Medical sciences; Platelet diseases and coagulopathies; Protein S Deficiency - diagnosis; Sensitivity and Specificity; Thrombomodulin; Thrombophilia - blood; Thrombophilia - diagnosis; Thromboplastin - pharmacology; Thromboplastin - standards; Human; Hypercoagulability; Deficiency; Cardiovascular disease; Hemopathy; Thrombosis; Factor VII; Vascular disease; Factor V; Thromboplastin; Factor X; Sensitivity; Prothrombin; Complication; Female; Protein S; Diagnosis; Technique; Thrombomodulin; Coagulation factor; Coagulopathy
• ISSN: 0390-6078; 1592-8721

Unitat d'Hemostasia i Trombosi, Hospital de la Santa Creu i Sant Pau, Avgda San Antoni M Claret 167, 08025 Barcelona, Spain. mborrell@hsp.santpau.es BACKGROUND AND OBJECTIVES: A new test for screening the procoagulant capacity of plasma is described and evaluated. This test is based on the coagulation of plasma initiated by thromboplastin (Tp) in the presence of thrombomodulin (TM). In a previous paper we reported that this test had a significant phenotypic and genetic correlation with thrombosis susceptibility. The present report describes the characteristics of the test and its sensitivity to the concentration of some hemostasis factors. DESIGN AND METHODS: Plasma from normal subjects, from individuals with various disorders of hemostasis and plasma with different concentrations of factors II, V, VII, VIII, X, fibrinogen, protein C and protein S were studied. The thromboplastin-thrombomodulin-mediated time (Tp-TMT) is measured after mixing 100 mL of plasma diluted 1/10 at 37 C with 100 mL of a solution composed of 2 parts of thromboplastin, 1 part of thrombomodulin at 30 U/mL and 1 part of Owren's buffer. The results are expressed as the ratio of the patient's clotting time to that of the control. Values were compared with Student's t test and the Mann-Whitney test. Differences were considered statistically significant when p