Identification of INSL6, a New Member of the Insulin Family That Is Expressed in the Testis of the Human and Rat
A new member of the insulin gene family ( INSL6 ) was identified from an Expressed Sequence Tag database through a search for proteins containing the insulin family B-chain cysteine motif. Human and rat INSL6 encoded polypeptides of 213 and 188 amino acids, respectively. These orthologous sequences...
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Published in | Biology of reproduction Vol. 62; no. 6; pp. 1593 - 1599 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Madison, WI
Society for the Study of Reproduction
01.06.2000
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Subjects | |
Online Access | Get full text |
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Summary: | A new member of the insulin gene family ( INSL6 ) was identified from an Expressed Sequence Tag database through a search for proteins containing the insulin family B-chain
cysteine motif. Human and rat INSL6 encoded polypeptides of 213 and 188 amino acids, respectively. These orthologous sequences contained the B-chain, C-peptide,
and A-chain motif found in other members of the insulin family. Human INSL6 was 43% identical to human relaxin H2 in the B-
and A-chain regions. As with other family members, human and rat INSL6 had predicted dibasic sequences at the junction of
the C-peptide and A-chain. Human INSL6 sequence had an additional dibasic site near the C-terminus of the A-chain. The presence
of a single basic residue at the predicted junction of the B-chain and C-peptide suggests that multiple prohormone convertases
are required to produce the fully mature hormone. INSL6 was found to be expressed at high levels in the testis as determined by Northern blot analysis and specifically within the
seminiferous tubules in spermatocytes and round spermatids as detected by in situ hybridization analysis. Radiation hybrid
mapping placed the human INSL6 locus at chromosome 9p24 near the placenta insulin-like homologue INSL4 and the autosomal testis-determining factor ( TDFA ) locus. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod62.6.1593 |