Pseudomonas aeruginosa protease IV produces corneal damage and contributes to bacterial virulence

• Published in: Investigative ophthalmology & visual science Vol. 39; no. 3; pp. 662 - 665
• Main Authors: Engel, LS, Hill, JM, Moreau, JM, Green, LC, Hobden, JA, O'Callaghan, RJ
• Format: Journal Article
• Language: English
• Published: Rockville, MD ARVO 01.03.1998; Association for Research in Vision and Ophtalmology
• Subjects: Animals; Bacterial diseases; Biological and medical sciences; Colony Count, Microbial; Cornea - drug effects; Cornea - microbiology; Cornea - pathology; Corneal Ulcer - chemically induced; Corneal Ulcer - microbiology; Corneal Ulcer - pathology; Experimental bacterial diseases and models; Eye Infections, Bacterial - chemically induced; Eye Infections, Bacterial - microbiology; Eye Infections, Bacterial - pathology; Infectious diseases; Medical sciences; Peptide Hydrolases - isolation & purification; Peptide Hydrolases - pharmacology; Pseudomonas aeruginosa - chemistry; Pseudomonas aeruginosa - enzymology; Pseudomonas aeruginosa - pathogenicity; Pseudomonas Infections - chemically induced; Pseudomonas Infections - microbiology; Pseudomonas Infections - pathology; Rabbits; Virulence; Pseudomonadales; Cornea; Keratitis; Pathogenesis; Virulence; Rabbit; Cytotoxicity; Keratopathy; Lagomorpha; Infection; Eye disease; Vertebrata; Experimental disease; Mammalia; Animal; Bacteriosis; Bacteria; Pseudomonadaceae; Pseudomonas aeruginosa

A Pseudomonas mutant deficient in protease IV has significantly reduced virulence in experimental keratitis. In the present study, the corneal toxicity of purified protease IV and its ability to augment the virulence of protease-IV-deficient bacteria were analyzed. The toxicity of purified protease...