Different Binding Modes of Amphibian and Human Corticotropin-Releasing Factor Type 1 and Type 2 Receptors: Evidence for Evolutionary Differences

• Published in: The Journal of pharmacology and experimental therapeutics Vol. 296; no. 1; pp. 113 - 120
• Main Authors: Dautzenberg, F M, Py-Lang, G, Higelin, J, Fischer, C, Wright, M B, Huber, G
• Format: Journal Article
• Language: English
• Published: United States American Society for Pharmacology and Experimental Therapeutics 01.01.2001
• Subjects: Animals; Binding, Competitive; Biological Evolution; Cell Line; Corticotropin-Releasing Hormone - analogs & derivatives; Corticotropin-Releasing Hormone - metabolism; Cyclic AMP - biosynthesis; Humans; Iodine Radioisotopes; Ligands; Peptides - metabolism; Radioligand Assay; Rats; Receptors, Corticotropin-Releasing Hormone - agonists; Receptors, Corticotropin-Releasing Hormone - antagonists & inhibitors; Receptors, Corticotropin-Releasing Hormone - metabolism; Xenopus

The binding characteristics of corticotropin-releasing factor (CRF) type 1 (CRF 1 ) and type 2 (CRF 2 ) receptors from human (hCRF 1 and hCRF 2α ) and Xenopus (xCRF 1 and xCRF 2 ) were compared using four different 125 I-labeled CRF analogs, the agonists 125 I-CRF and 125 I-sauvagine, and the antag...