In Transduced Cells, the US3 Protein Kinase of Herpes Simplex Virus 1 Precludes Activation and Induction of Apoptosis by Transfected Procaspase 3

Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from...

Full description

Saved in:
Bibliographic Details
Published inJournal of Virology Vol. 81; no. 19; pp. 10242 - 10248
Main Authors BENETTI, Luca, ROIZMAN, Bernard
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.10.2007
Subjects
Animals
Apoptosis
Biological and medical sciences
Caspase 3 - genetics
Caspase 3 - metabolism
Caspase Inhibitors
Cell Line, Tumor
Enzyme Induction
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Miscellaneous
Phosphorylation
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Transduction, Genetic
Transfection
Viral Proteins - genetics
Viral Proteins - metabolism
Virology
Virus-Cell Interactions
Enzyme
Induction
Herpesviridae
Transferases
Alphaherpesvirinae
In vitro
Virology
Virus
Signal transduction
Cell death
Protein kinase
Human herpesvirus 1
Apoptosis
Online AccessGet full text

Cover

Abstract Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JVI .asm.org, visit: JVI       
AbstractList The U S 3 protein kinase of herpes simplex virus 1 blocks apoptosis induced by replication-incompetent virus mutants, proapoptotic members of the Bcl-2 family of proteins, and by a variety of other agents that act at the premitochondrial level in the proapoptotic cascade. To define the role of U S 3 in blocking apoptosis at the postmitochondrial level, we investigated the U S 3 protein kinase in transduced cells that were either transfected with a plasmid encoding procaspase 3 or superinfected with a proapoptotic mutant virus lacking the gene encoding the infected cell protein no. 4. (i) We show that U S 3 blocks the proteolytic cleavage that generates active caspase 3 from the transfected zymogen procaspase 3, concomitant with inhibition of apoptosis. (ii) Studies based on detection of fluorescence emitted upon cleavage of a synthetic caspase 3 substrate showed that expression of the U S 3 kinase and appearance of the cleaved substrate were mutually exclusive. (iii) An affinity-purified glutathione S -transferase (GST)-U S 3 fusion protein, but not the inactive GST-U S 3(K220N) protein, phosphorylated procaspase 3 in vitro. The studies published earlier on the effect of U S 3 on the upstream regulatory proteins and current studies suggest that the U S 3 protein kinase may act on several proteins in the proapoptotic cascade to enable the virus to complete its replication.
Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JVI .asm.org, visit: JVI       
The US3 protein kinase of herpes simplex virus 1 blocks apoptosis induced by replication-incompetent virus mutants, proapoptotic members of the Bcl-2 family of proteins, and by a variety of other agents that act at the premitochondrial level in the proapoptotic cascade. To define the role of US3 in blocking apoptosis at the postmitochondrial level, we investigated the US3 protein kinase in transduced cells that were either transfected with a plasmid encoding procaspase 3 or superinfected with a proapoptotic mutant virus lacking the gene encoding the infected cell protein no. 4. (i) We show that US3 blocks the proteolytic cleavage that generates active caspase 3 from the transfected zymogen procaspase 3, concomitant with inhibition of apoptosis. (ii) Studies based on detection of fluorescence emitted upon cleavage of a synthetic caspase 3 substrate showed that expression of the US3 kinase and appearance of the cleaved substrate were mutually exclusive. (iii) An affinity-purified glutathione S-transferase (GST)-US3 fusion protein, but not the inactive GST-US3(K220N) protein, phosphorylated procaspase 3 in vitro. The studies published earlier on the effect of US3 on the upstream regulatory proteins and current studies suggest that the US3 protein kinase may act on several proteins in the proapoptotic cascade to enable the virus to complete its replication.
Author Luca Benetti
Bernard Roizman
AuthorAffiliation Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Chicago, Illinois 60637
AuthorAffiliation_xml – name: Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Chicago, Illinois 60637
Author_xml – sequence: 1
  givenname: Luca
  surname: BENETTI
  fullname: BENETTI, Luca
  organization: Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Chicago, Illinois 60637, United States
– sequence: 2
  givenname: Bernard
  surname: ROIZMAN
  fullname: ROIZMAN, Bernard
  organization: Marjorie B. Kovler Viral Oncology Laboratories, University of Chicago, Chicago, Illinois 60637, United States
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19076026$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/17634220$$D View this record in MEDLINE/PubMed
BookMark eNpVkU1v1DAQhi1URLeFG2dkCcGJFNvxR3KptFoBXagEUj_EzXLsSeMqa4c42bY_g3-MYZev02g0z7yPNHOEDkIMgNBzSk4oZdXbj9frE0IqRgqiHqEFJXVVCEH5AVoQwlghyurrITpK6ZYQyrnkT9AhVbLkjJEF-r4O-HI0IbnZgsMr6Pv0Bk8d4KuLEn8Z4wQ-4E8-mAQ4tvgMxgESvvCboYd7fO3HOWGaQbD97PJkaSe_NZOPAZvg8Drk4F9dXl4OcZhi8gk3DztrC3bK2uyxJg0_HeVT9Lg1fYJn-3qMrt6_u1ydFeefP6xXy_OiY6WailI42daOq4apSlS2haYmXElXSldz2xBRKbBGARAraua4bEgthSRO8JI6Wx6j013uMDcbcBbCNJpeD6PfmPFBR-P1_5PgO30Tt5oRLnitcsDrfcAYv82QJr3xyeYDmgBxTlpWTHFZiQy--Nf0R_H7Cxl4tQdMsqZv82WsT3-5mihJmMzcyx3X-Zvuzo-gTdro263XFc2UpoRxVv4AZ4qlKw
ContentType Journal Article
Copyright 2007 INIST-CNRS
Copyright © 2007, American Society for Microbiology 2007
Copyright_xml – notice: 2007 INIST-CNRS
– notice: Copyright © 2007, American Society for Microbiology 2007
DBID IQODW
CGR
CUY
CVF
ECM
EIF
NPM
7X8
5PM
DOI 10.1128/JVI.00820-07
DatabaseName Pascal-Francis
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
MEDLINE - Academic
DatabaseTitleList

MEDLINE - Academic
MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1098-5514
EndPage 10248
ExternalDocumentID 17634220
19076026
jvi_81_19_10242
Genre Journal Article
Research Support, N.I.H., Extramural
GrantInformation_xml – fundername: NCI NIH HHS
  grantid: CA71933
– fundername: NCI NIH HHS
  grantid: R01 CA078766
– fundername: NCI NIH HHS
  grantid: R01 CA115662
– fundername: NCI NIH HHS
  grantid: CA83939
– fundername: NCI NIH HHS
  grantid: CA78766
– fundername: NCI NIH HHS
  grantid: R01 CA088860
– fundername: NCI NIH HHS
  grantid: CA88860
– fundername: NCI NIH HHS
  grantid: R37 CA078766
– fundername: NCI NIH HHS
  grantid: R01 CA083939
– fundername: NCI NIH HHS
  grantid: CA115662
GroupedDBID ---
-~X
.55
.GJ
08R
0R~
18M
29L
2WC
39C
3O-
4.4
41~
53G
5GY
5RE
5VS
6TJ
85S
AAUGY
AAYJJ
ABPPZ
ABPTK
ACGFO
ACNCT
ADBBV
AENEX
AFDAS
AFFNX
AI.
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BTFSW
C1A
CS3
D0S
DIK
E3Z
EBS
EJD
F20
F5P
FRP
GX1
H13
HYE
HZ~
IH2
IQODW
KQ8
MVM
N9A
O9-
OHT
OK1
P2P
RHF
RHI
RNS
RPM
RSF
TR2
UCJ
UPT
VH1
W2D
W8F
WH7
WOQ
X7M
XFK
Y6R
YQT
ZA5
ZGI
ZXP
~02
~KM
AGVNZ
CGR
CUY
CVF
ECM
EIF
NPM
7X8
5PM
ID FETCH-LOGICAL-h237t-35d6f9d47b27858cfeb90476d36d94cb0587eca7ee0c592d46b096560d5431dc3
IEDL.DBID RPM
ISSN 0022-538X
IngestDate Tue Sep 17 21:24:50 EDT 2024
Fri Aug 16 22:03:04 EDT 2024
Sat Sep 28 07:43:35 EDT 2024
Sun Oct 22 16:03:44 EDT 2023
Wed May 18 15:33:06 EDT 2016
IsPeerReviewed true
IsScholarly true
Issue 19
Keywords Enzyme
Induction
Herpesviridae
Transferases
Alphaherpesvirinae
In vitro
Virology
Virus
Signal transduction
Cell death
Protein kinase
Human herpesvirus 1
Apoptosis
Language English
License CC BY 4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-h237t-35d6f9d47b27858cfeb90476d36d94cb0587eca7ee0c592d46b096560d5431dc3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Corresponding author. Mailing address: The Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, 910 East 58th Street, Chicago, IL 60637. Phone: (773) 702-1898. Fax: (773) 702-1631. E-mail: bernard.roizman@bsd.uchicago.edu
PMID 17634220
PQID 68274685
PQPubID 23479
PageCount 7
ParticipantIDs pubmed_primary_17634220
proquest_miscellaneous_68274685
highwire_asm_jvi_81_19_10242
pubmedcentral_primary_oai_pubmedcentral_nih_gov_2045497
pascalfrancis_primary_19076026
PublicationCentury 2000
PublicationDate 2007-10-00
PublicationDateYYYYMMDD 2007-10-01
PublicationDate_xml – month: 10
  year: 2007
  text: 2007-10-00
PublicationDecade 2000
PublicationPlace Washington, DC
PublicationPlace_xml – name: Washington, DC
– name: United States
PublicationTitle Journal of Virology
PublicationTitleAlternate J Virol
PublicationYear 2007
Publisher American Society for Microbiology
Publisher_xml – name: American Society for Microbiology
References 12743316 - J Virol. 2003 Jun;77(11):6567-73
16785443 - Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9993-8
9748481 - Biochim Biophys Acta. 1998 Sep 8;1387(1-2):17-31
10611736 - Microbes Infect. 1999 Jul;1(8):601-7
10074128 - J Virol. 1999 Apr;73(4):2803-13
16537601 - J Virol. 2006 Apr;80(7):3341-8
11090178 - J Virol. 2000 Dec;74(24):11782-91
8790373 - Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9583-7
9520421 - Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3655-60
7796396 - Cancer Res. 1995 Jul 1;55(13):2737-42
14610189 - J Virol. 2003 Dec;77(23):12671-8
10066732 - J Biol Chem. 1999 Mar 12;274(11):6823-6
11356956 - J Virol. 2001 Jun;75(12):5491-7
9747872 - Oncogene. 1998 Aug 27;17(8):931-9
10872455 - Annu Rev Biochem. 1999;68:383-424
14970175 - J Exp Med. 2004 Feb 16;199(4):449-58
14980482 - Virology. 2004 Feb 20;319(2):212-24
16921264 - Cancer Biol Ther. 2006 Jul;5(7):763-5
14597423 - Exp Cell Res. 2003 Nov 15;291(1):242-50
15192152 - Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9411-6
3039176 - J Virol. 1987 Sep;61(9):2896-901
11114501 - Curr Opin Struct Biol. 2000 Dec;10(6):649-55
1809356 - Annu Rev Cell Biol. 1991;7:663-98
16571792 - J Virol. 2006 Apr;80(8):3752-64
9520470 - Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3931-6
10200555 - Cell Death Differ. 1999 Feb;6(2):99-104
8521391 - Cancer Res. 1995 Dec 15;55(24):6045-52
16469926 - Science. 2006 Feb 10;311(5762):847-51
10644366 - J Virol. 2000 Feb;74(4):1931-8
10074175 - J Virol. 1999 Apr;73(4):3219-26
15956590 - J Virol. 2005 Jul;79(13):8470-9
9223283 - Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):7891-6
11517326 - Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10410-5
11170436 - Biochemistry. 2001 Jan 30;40(4):1117-23
12163613 - J Virol. 2002 Sep;76(17):8939-52
10516000 - J Virol. 1999 Nov;73(11):8950-7
11773417 - J Virol. 2002 Feb;76(3):1435-49
9850056 - Cancer Res. 1998 Dec 1;58(23):5315-20
9032402 - J Virol. 1997 Mar;71(3):2567-71
11287608 - Mol Cell Biol. 2001 May;21(9):3025-36
11564811 - J Immunol. 2001 Oct 1;167(7):3928-35
17409148 - J Virol. 2007 Jun;81(12):6316-25
References_xml
SSID ssj0014464
Score 2.1026728
Snippet Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
The US3 protein kinase of herpes simplex virus 1 blocks apoptosis induced by replication-incompetent virus mutants, proapoptotic members of the Bcl-2 family of...
The U S 3 protein kinase of herpes simplex virus 1 blocks apoptosis induced by replication-incompetent virus mutants, proapoptotic members of the Bcl-2 family...
SourceID pubmedcentral
proquest
pubmed
pascalfrancis
highwire
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 10242
SubjectTerms Animals
Apoptosis
Biological and medical sciences
Caspase 3 - genetics
Caspase 3 - metabolism
Caspase Inhibitors
Cell Line, Tumor
Enzyme Induction
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Miscellaneous
Phosphorylation
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Transduction, Genetic
Transfection
Viral Proteins - genetics
Viral Proteins - metabolism
Virology
Virus-Cell Interactions
Title In Transduced Cells, the US3 Protein Kinase of Herpes Simplex Virus 1 Precludes Activation and Induction of Apoptosis by Transfected Procaspase 3
URI http://jvi.asm.org/content/81/19/10242.abstract
https://www.ncbi.nlm.nih.gov/pubmed/17634220
https://search.proquest.com/docview/68274685
https://pubmed.ncbi.nlm.nih.gov/PMC2045497
Volume 81
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1db9MwFLW2SUi8IL4JG8UPPJI2jhM7eawqpnbT0KTRqW-Rv6IFtU7UtGj7Gfxjrp1kUMQTz7ZlK9f2vTc-9xyEPsU5N1JDpiolgQQljUq4B6kOtaMTl4KS0uunXH1l82VysUpXRygdamE8aF_JamzXm7Gt7jy2stmoyYATm1xfzRyFepLzyTE65pQOKXr_dAD5TTJQhMNpXg1o9zibXNwuxt7nhZEX34OzlcRO6fuRG9hBI0ULX6fsZC3-FXf-DZ_8wx-dP0fP-kAST7sFv0BHxr5ETzppyYdX6OfCYu-HNNhO45lZr9vPGKI9vLyh-NqxM1QWX1YWvBiuSzw328a0-KZybMH3-Lba7ltMoKNR672GlqkahNCwsBo7yQ9fEuEGT5u62dVt1WL50M3qQCIwrStDEHBnwRz0NVqef_k2m4e9_EJ4F1O-C2mqWZnrhMuYZ2mmSiPzKOFMU6bzRMkozbhRghsTqTSPdcKko5JhkXb19VrRN-jE1ta8Q1gplQnCy4wKyJBkLnOtJYkJ00IluiQBOhssUIh2U3z_URUZKUheEBdDBGh0YJWi6Zg4oIN7WIxZgD4OZirgeLg3D2FNvW8LlkHazbI0QG87o_0e21s_QPzAnI8dHPH2YQvsR0_A3e-_9_898hQ99f-IPSjwDJ3stnvzAYKbnRxBWL-4HPkt_QuXkPwR
link.rule.ids 230,315,733,786,790,891,27957,27958,53827,53829
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELZKEYIL70KgtD5wJNk87eS4WlHttt2qUrurvUXxI2pg14k2CaL8C_4xYycpbMUFzrZlOTPjmYm_-Qahj35CJROQqTLmQYISuTncg4GwhaYTZ1ng5aZ_yvyCTBfh6Spa7aFoqIUxoH3OCketN44qbgy2strw0YATG13OJ5pCPUzo6AF6CPbq0yFJ7x8PIMMJB5JwGF8NeHc_Hp0uZ47xerZr2u-BdYW-7vV9xw6swZFZDd8n7xpb_C3yvA-g_MMjnTxDy-EsHRDlq9M2zOE_7tE8_vNhn6OnfYyKx93wC7Qn1Uv0qOtaefsK_ZwpbFycALUQeCLX6_oThkASL64CfKmJHwqFzwoFDhKXOZ7KbSVrfFVoIuLveFls2xp7MFHydStgZMyHHms4UwLrbiKm2kIvHldl1ZR1UWN22-2q8Sewra5wyOA6hD2C12hx8vl6MrX7zg72jR_Qxg4iQfJEhJT5NI5inkuWuCElIiAiCTlzo5hKnlEpXR4lvggJ0yw1xBW6dF_w4ADtq1LJtwhzzuPMo3kcZJB8sYQlQjDP94jIeChyz0KHg2jTrN6kX74VaeylXpJ6Ojyx0NGOuNOqI_mACfrN0icWOh7kn4Ll6eeUTMmyrVMCmheSOLLQm04bfq_t1cpCdEdP7iZoTu_dEZC-4fbupf3uv1ceo8fT6_l5ej67OHuPnphf0QZ7eIj2m20rP0AM1bAjYzG_ACwBHR4
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwELagCMSFdyFQWh84kmyednJcLax2W1qtVLZacYniR9TArhNtEkT5F_xjxk5SuhWnnj2W5XjGnok_fx9CH_yESiagUmXMgwIlcnPYBwNhC00nzrLAy41-yukZmS3D41W0uiH1ZUD7nBWOWm8cVVwabGW14aMBJzZanE40hXqY0FEl8tF99ABi1k-GQr2_QIAqJxyIwqF9NWDe_Xh0fDF3zMlnu0aCDyIs9LXe9zVDsAZIZjV8o7wTt_hf9nkbRHnjVJo-Rd-G-XRglB9O2zCH_75F9XinCT9DT_pcFY87k-fonlQv0MNOvfLqJfozV9gcdQLcQ-CJXK_rjxgSSrw8D_BCE0AUCp8UCg5KXOZ4JreVrPF5oQmJf-GLYtvW2ANDydetgJYxH7TWcKYE1qoi5tWF7jyuyqop66LG7KobVeNQYFj90iGDbRHGCF6h5fTz18nM7hUe7Es_oI0dRILkiQgp82kcxTyXLHFDSkRARBJy5kYxlTyjUro8SnwREqbZaogr9BN-wYN9tKdKJd8gzDmPM4_mcZBBEcYSlgjBPN8jIuOhyD0LHQzLm2b1Jv3-s0hjL_WS1NNpioUOd5Y8rTqyDzDQd5c-sdDR4AMpRKC-VsmULNs6JTFU9iSOLPS684h_fXvXshDd8ZVrA83tvdsCHmA4vvsVf3vnnkfo0eLTNP0yPzt5hx6bP9IGgniA9pptK99DKtWwQxM0fwEFkx-e
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In+transduced+cells%2C+the+US3+protein+kinase+of+herpes+simplex+virus+1+precludes+activation+and+induction+of+apoptosis+by+transfected+procaspase+3&rft.jtitle=Journal+of+virology&rft.au=Benetti%2C+Luca&rft.au=Roizman%2C+Bernard&rft.date=2007-10-01&rft.issn=0022-538X&rft.volume=81&rft.issue=19&rft.spage=10242&rft.epage=10248&rft_id=info:doi/10.1128%2FJVI.00820-07&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-538X&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-538X&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-538X&client=summon