Hyperthermia stimulates plasminogen activator inhibitor type 1 expression in human umbilical vein endothelial cells in vitro

• Published in: The American journal of pathology Vol. 139; no. 4; pp. 911 - 919
• Main Authors: Wojta, J, Holzer, M, Hufnagl, P, Christ, G, Hoover, RL, Binder, BR
• Format: Journal Article
• Language: English
• Published: Bethesda, MD ASIP 01.10.1991; American Society for Investigative Pathology
• Subjects: Biological and medical sciences; Blotting, Northern; Cell physiology; Cells, Cultured; Effects of physical and chemical agents; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Extracellular Matrix - metabolism; Fever - physiopathology; Fibrinolysis - physiology; Fundamental and applied biological sciences. Psychology; Gene Expression; Humans; Membrane Proteins - genetics; Membrane Proteins - metabolism; Molecular and cellular biology; Peroxisomal Biogenesis Factor 2; RNA, Messenger - genetics; RNA, Messenger - metabolism; Time Factors; Umbilical Veins - cytology; Human origin; Cell culture; Endothelial cell; Enzyme; Cell biology; Plasminogen activator; Fibrinolysis; In vitro; Time dependence; Biological effect; Inhibition; Hyperthermia; Umbilical vein
• ISSN: 0002-9440; 1525-2191

The effect of exposure to hyperthermia on the fibrinolytic potential of human umbilical vein endothelial cells (HUVEC) in culture was studied. HUVEC responded to exposure to 42 degrees C with a time-dependent increase in plasminogen activator inhibitor type 1 (PAI-1) activity and antigen accompanied by a four- to fivefold increase in PAI-1 specific m-RNA and a decrease in tissue-type plasminogen activator (t-PA) antigen. The effect of 8 hours exposure to hyperthermia on PAI-1 activity and antigen could not be reversed by reexposure of the cells to 37 degrees C for 24 hours as evidenced by continuously increased amounts of PAI-1 released into the conditioned media. t-PA release, however, decreased during the 24-hour period at 37 degrees C after exposure to hyperthermia. No difference in PAI-1 antigen present in the extracellular matrix of heat treated HUVEC as compared to HUVEC kept at 37 degrees C could be found. Our data supports the idea that hyperthermia is one stress factor that influences the fibrinolytic potential of endothelial cells.