Multidrug resistance phenotype of human BRO melanoma cells transfected with a wild-type human mdr1 complementary DNA

• Published in: Cancer research (Chicago, Ill.) Vol. 50; no. 6; pp. 1779 - 1785
• Main Authors: LINCKE, C. R, VAN DER BLIEK, A. M, SCHUURHUIS, G. J, VAN DER VELDE-KOERTS, T, SMIT, J. J. M, BORST, P
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.03.1990
• Subjects: Antineoplastic agents; Antineoplastic Agents - pharmacology; ATP-Binding Cassette, Sub-Family B, Member 1; Biological and medical sciences; Biological Transport; BRO cells; Cell Division - drug effects; Cell Line; Cell Membrane - metabolism; Daunorubicin - metabolism; DNA - genetics; drug resistance; Drug Resistance - genetics; General aspects; Humans; Kinetics; Medical sciences; Melanoma; Membrane Glycoproteins - genetics; Nucleic Acid Hybridization; Pharmacology. Drug treatments; Phenotype; Transfection; Tumor Cells, Cultured - cytology; Tumor Cells, Cultured - drug effects; Tumor Cells, Cultured - metabolism; Antineoplastic agent; Human; Cell line; Sensitivity resistance; Transfection; Melanoma; Tumor; Gene expression

We have transfected a eukaryotic expression vector containing a mdr1 complementary DNA isolated from normal human liver into human BRO melanoma cells to study the drug-resistant phenotype produced by the exclusive overexpression of normal human mdr1 P-glycoprotein. The drug resistance pattern of mdr...