The Genome Sequence of Methanosphaera stadtmanae Reveals Why This Human Intestinal Archaeon Is Restricted to Methanol and H2 for Methane Formation and ATP Synthesis
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| Published in | Journal of Bacteriology Vol. 188; no. 2; pp. 642 - 658 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
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United States
American Society for Microbiology
01.01.2006
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| AbstractList | Methanosphaera stadtmanae
has the most restricted energy metabolism of all methanogenic archaea. This human intestinal inhabitant can generate methane only by reduction of methanol with H
2
and is dependent on acetate as a carbon source. We report here the genome sequence of
M. stadtmanae
, which was found to be composed of 1,767,403 bp with an average G+C content of 28% and to harbor only 1,534 protein-encoding sequences (CDS). The genome lacks 37 CDS present in the genomes of all other methanogens. Among these are the CDS for synthesis of molybdopterin and for synthesis of the carbon monoxide dehydrogenase/acetyl-coenzyme A synthase complex, which explains why
M. stadtmanae
cannot reduce CO
2
to methane or oxidize methanol to CO
2
and why this archaeon is dependent on acetate for biosynthesis of cell components. Four sets of
mtaABC
genes coding for methanol:coenzyme M methyltransferases were found in the genome of
M. stadtmanae
. These genes exhibit homology to
mta
genes previously identified in
Methanosarcina
species. The
M. stadtmanae
genome also contains at least 323 CDS not present in the genomes of all other archaea. Seventy-three of these CDS exhibit high levels of homology to CDS in genomes of bacteria and eukaryotes. These 73 CDS include 12 CDS which are unusually long (>2,400 bp) with conspicuous repetitive sequence elements, 13 CDS which exhibit sequence similarity on the protein level to CDS encoding enzymes involved in the biosynthesis of cell surface antigens in bacteria, and 5 CDS which exhibit sequence similarity to the subunits of bacterial type I and III restriction-modification systems. Methanosphaera stadtmanae has the most restricted energy metabolism of all methanogenic archaea. This human intestinal inhabitant can generate methane only by reduction of methanol with H2 and is dependent on acetate as a carbon source. We report here the genome sequence of M. stadtmanae, which was found to be composed of 1,767,403 bp with an average G+C content of 28% and to harbor only 1,534 protein-encoding sequences (CDS). The genome lacks 37 CDS present in the genomes of all other methanogens. Among these are the CDS for synthesis of molybdopterin and for synthesis of the carbon monoxide dehydrogenase/acetyl-coenzyme A synthase complex, which explains why M. stadtmanae cannot reduce CO2 to methane or oxidize methanol to CO2 and why this archaeon is dependent on acetate for biosynthesis of cell components. Four sets of mtaABC genes coding for methanol:coenzyme M methyltransferases were found in the genome of M. stadtmanae. These genes exhibit homology to mta genes previously identified in Methanosarcina species. The M. stadtmanae genome also contains at least 323 CDS not present in the genomes of all other archaea. Seventy-three of these CDS exhibit high levels of homology to CDS in genomes of bacteria and eukaryotes. These 73 CDS include 12 CDS which are unusually long (>2,400 bp) with conspicuous repetitive sequence elements, 13 CDS which exhibit sequence similarity on the protein level to CDS encoding enzymes involved in the biosynthesis of cell surface antigens in bacteria, and 5 CDS which exhibit sequence similarity to the subunits of bacterial type I and III restriction-modification systems. Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JB About JB Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JB RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0021-9193 Online ISSN: 1098-5530 Copyright © 2014 by the American Society for Microbiology. For an alternate route to JB .asm.org, visit: JB Methanosphaera stadtmanae has the most restricted energy metabolism of all methanogenic archaea. This human intestinal inhabitant can generate methane only by reduction of methanol with H2 and is dependent on acetate as a carbon source. We report here the genome sequence of M. stadtmanae, which was found to be composed of 1,767,403 bp with an average G+C content of 28% and to harbor only 1,534 protein-encoding sequences (CDS). The genome lacks 37 CDS present in the genomes of all other methanogens. Among these are the CDS for synthesis of molybdopterin and for synthesis of the carbon monoxide dehydrogenase/acetyl-coenzyme A synthase complex, which explains why M. stadtmanae cannot reduce CO2 to methane or oxidize methanol to CO2 and why this archaeon is dependent on acetate for biosynthesis of cell components. Four sets of mtaABC genes coding for methanol:coenzyme M methyltransferases were found in the genome of M. stadtmanae. These genes exhibit homology to mta genes previously identified in Methanosarcina species. The M. stadtmanae genome also contains at least 323 CDS not present in the genomes of all other archaea. Seventy-three of these CDS exhibit high levels of homology to CDS in genomes of bacteria and eukaryotes. These 73 CDS include 12 CDS which are unusually long (>2,400 bp) with conspicuous repetitive sequence elements, 13 CDS which exhibit sequence similarity on the protein level to CDS encoding enzymes involved in the biosynthesis of cell surface antigens in bacteria, and 5 CDS which exhibit sequence similarity to the subunits of bacterial type I and III restriction-modification systems.Methanosphaera stadtmanae has the most restricted energy metabolism of all methanogenic archaea. This human intestinal inhabitant can generate methane only by reduction of methanol with H2 and is dependent on acetate as a carbon source. We report here the genome sequence of M. stadtmanae, which was found to be composed of 1,767,403 bp with an average G+C content of 28% and to harbor only 1,534 protein-encoding sequences (CDS). The genome lacks 37 CDS present in the genomes of all other methanogens. Among these are the CDS for synthesis of molybdopterin and for synthesis of the carbon monoxide dehydrogenase/acetyl-coenzyme A synthase complex, which explains why M. stadtmanae cannot reduce CO2 to methane or oxidize methanol to CO2 and why this archaeon is dependent on acetate for biosynthesis of cell components. Four sets of mtaABC genes coding for methanol:coenzyme M methyltransferases were found in the genome of M. stadtmanae. These genes exhibit homology to mta genes previously identified in Methanosarcina species. The M. stadtmanae genome also contains at least 323 CDS not present in the genomes of all other archaea. Seventy-three of these CDS exhibit high levels of homology to CDS in genomes of bacteria and eukaryotes. These 73 CDS include 12 CDS which are unusually long (>2,400 bp) with conspicuous repetitive sequence elements, 13 CDS which exhibit sequence similarity on the protein level to CDS encoding enzymes involved in the biosynthesis of cell surface antigens in bacteria, and 5 CDS which exhibit sequence similarity to the subunits of bacterial type I and III restriction-modification systems. |
| Author | Rudolf K. Thauer Reiner Hedderich Markus Krüer Wolfgang F. Fricke Gerhard Gottschalk Heiko Liesegang Henning Seedorf Anke Henne |
| AuthorAffiliation | Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, 1 Department of Biochemistry, 2 Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany 3 |
| AuthorAffiliation_xml | – name: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, 1 Department of Biochemistry, 2 Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany 3 |
| Author_xml | – sequence: 1 givenname: Wolfgang F. surname: Fricke fullname: Fricke, Wolfgang F. organization: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, Department of Biochemistry, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany – sequence: 2 givenname: Henning surname: Seedorf fullname: Seedorf, Henning organization: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, Department of Biochemistry, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany – sequence: 3 givenname: Anke surname: Henne fullname: Henne, Anke organization: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, Department of Biochemistry, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany – sequence: 4 givenname: Markus surname: Krüer fullname: Krüer, Markus organization: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, Department of Biochemistry, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany – sequence: 5 givenname: Heiko surname: Liesegang fullname: Liesegang, Heiko organization: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, Department of Biochemistry, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany – sequence: 6 givenname: Reiner surname: Hedderich fullname: Hedderich, Reiner organization: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, Department of Biochemistry, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany – sequence: 7 givenname: Gerhard surname: Gottschalk fullname: Gottschalk, Gerhard email: ggottsc@gwdg.de organization: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, Department of Biochemistry, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany – sequence: 8 givenname: Rudolf K. surname: Thauer fullname: Thauer, Rudolf K. organization: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, 37077 Göttingen, Germany, Department of Biochemistry, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16385054$$D View this record in MEDLINE/PubMed |
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| DOI | 10.1128/JB.188.2.642-658.2006 |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 W.F.F. and H.S. contributed equally to this work. Present address: Qiagen AG, Hilden, Germany. Corresponding author. Mailing address: Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg August University, Grisebachstr. 8, D-37077 Göttingen, Germany. Phone: 49-551-394041. Fax: 49-551-394195. E-mail: ggottsc@gwdg.de. |
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Mendeley... Methanosphaera stadtmanae has the most restricted energy metabolism of all methanogenic archaea. This human intestinal inhabitant can generate methane only by... Methanosphaera stadtmanae has the most restricted energy metabolism of all methanogenic archaea. This human intestinal inhabitant can generate methane only by... |
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| SubjectTerms | Adenosine Triphosphate - biosynthesis Aldehyde Oxidoreductases - biosynthesis Aldehyde Oxidoreductases - genetics Base Composition Coenzymes Deuterium - metabolism Genome, Archaeal Genomics and Proteomics Metalloproteins Methane - biosynthesis Methanobacteriaceae - genetics Methanobacteriaceae - growth & development Methanobacteriaceae - metabolism Methanol - metabolism Molecular Sequence Data Molybdenum Cofactors Multienzyme Complexes - biosynthesis Multienzyme Complexes - genetics Organometallic Compounds - metabolism Proteome - genetics Pteridines - metabolism |
| Title | The Genome Sequence of Methanosphaera stadtmanae Reveals Why This Human Intestinal Archaeon Is Restricted to Methanol and H2 for Methane Formation and ATP Synthesis |
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