Mu and delta opioid receptor desensitization in undifferentiated human neuroblastoma SHSY5Y cells

• Published in: The Journal of pharmacology and experimental therapeutics Vol. 270; no. 1; pp. 177 - 184
• Main Authors: Prather, P L, Tsai, A W, Law, P Y
• Format: Journal Article
• Language: English
• Published: United States American Society for Pharmacology and Experimental Therapeutics 01.07.1994
• Subjects: Adenylyl Cyclase Inhibitors; Adenylyl Cyclases - metabolism; Analgesics - pharmacology; Cell Differentiation - physiology; Colforsin - antagonists & inhibitors; Colforsin - pharmacology; Cyclic AMP - biosynthesis; Endorphins - pharmacology; Enkephalin, D-Penicillamine (2,5); Enkephalins - pharmacology; Humans; Neuroblastoma; Neurons - drug effects; Neurons - physiology; Neurons - ultrastructure; Receptors, Opioid, delta - drug effects; Receptors, Opioid, delta - metabolism; Receptors, Opioid, delta - physiology; Receptors, Opioid, mu - drug effects; Receptors, Opioid, mu - metabolism; Receptors, Opioid, mu - physiology; Sensitivity and Specificity; Signal Transduction - physiology; Stimulation, Chemical; Tumor Cells, Cultured

Both mu and delta opioid receptors are expressed in undifferentiated human neuroblastoma SHSY5Y cells and are negatively coupled to adenylate cyclase. The ability of various mu opioid, delta opioid and alpha-2 adrenergic agonists to inhibit acutely forskolin-stimulated adenylate cyclase activity in...