Amino acid sequence of the alpha and beta subunits of Methanosarcina barkeri ATPase deduced from cloned genes. Similarity to subunits of eukaryotic vacuolar and F0F1-ATPases

• Published in: The Journal of biological chemistry Vol. 264; no. 19; pp. 10954 - 10959
• Main Authors: KEN-ICHI INATOMI, SEIJI EYA, MAEDA, M, FUTAI, M
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 05.07.1989
• Subjects: Adenosine Triphosphatases - genetics; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Base Sequence; Biological and medical sciences; Cloning, Molecular; Enzymes and enzyme inhibitors; Escherichia coli - enzymology; Euryarchaeota - enzymology; Euryarchaeota - genetics; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Gram-Negative Chemolithotrophic Bacteria - enzymology; Hydrolases; Molecular Sequence Data; Neurospora crassa - enzymology; Oxidative Phosphorylation Coupling Factors; Plants - enzymology; Proton-Translocating ATPases; Saccharomyces cerevisiae - enzymology; Sequence Homology, Nucleic Acid; Vacuoles - enzymology; Methanomicrobiales; Molecular hybridization; Eucaryote; Methanosarcina barkeri; Nucleotide sequence; Archaeobacteria; ATPase; Bacteria; Methanosarcinaceae; Homology; Molecular cloning; Subunit

The atpA and atpB genes coding for the alpha and beta subunits, respectively, of membrane ATPase were cloned from a methanogen Methanosarcina barkeri, and the amino acid sequences of the two subunits were deduced from the nucleotide sequences. The methanogenic alpha (578 amino acid residues) and bet...