Insights into the Structure and Dynamics of Measles Virus Nucleocapsids by (1)H-detected Solid-state NMR

• Published in: Biophysical journal Vol. 107; no. 4; pp. 941 - 946
• Main Authors: Barbet-Massin, Emeline, Felletti, Michele, Schneider, Robert, Jehle, Stefan, Communie, Guillaume, Martinez, Nicolas, Jensen, Malene Ringkjøbing, Ruigrok, Rob W H, Emsley, Lyndon, Lesage, Anne, Blackledge, Martin, Pintacuda, Guido
• Format: Journal Article
• Language: English
• Published: Biophysical Society 19.08.2014
• Subjects: Analytical chemistry; Chemical Sciences; ANGLE-SPINNING NMR; AMYLOID FIBRILS; SPECTROSCOPY; RNA; NUCLEOPROTEIN; RESOLUTION; C-TERMINAL DOMAIN; CAPSID PROTEIN ASSEMBLIES; MEMBRANE-PROTEINS; BINDING

(1)H-detected solid-state nuclear magnetic resonance (NMR) experiments are recorded on both intact and trypsin-cleaved sedimented measles virus (MeV) nucleocapsids under ultra-fast magic-angle spinning. High-resolution (1)H,(15)N-fingerprints allow probing the degree of molecular order and flexibili...