Circulacion de Streptococcus neumoniaeclon [Colombia.sup.5] ST289 en nueve paises de America Latina

• Published in: Revista panamericana de salud pública Vol. 25; no. 4; p. 337
• Main Authors: Firacative, Carolina, Moreno, Jaime, Rosales, Patricia, Maldonado, Aurora, Sanchez, Jacqueline, Pesantes, Carmen, Lopez, Sergio, de Quinzada, Markela, Chamorro, Gustavo, Morales, Sara, Spadola, Enza, Gabastou, Jean-Marc, Castaneda, 11 and Elizabeth
• Format: Journal Article
• Language: Spanish
• Published: Pan American Health Organization 01.04.2009
• ISSN: 1020-4989

Objetivo. Determinar la relacion genetica del clon Colombia5 ST289 con los aislamientos invasores de Streptococcus pneumoniae serotipo 5 provenientes de nueve paises latinoamericanos. Metodos. Se estudiaron 45 aislamientos invasores de Streptococcus pneumoniae serotipo 5 procedentes de ninos menores de 5 anos de Bolivia, Chile, Ecuador, Nicaragua, Panama, Paraguay, Peru, Republica Dominicana y Venezuela. Los patrones en electroforesis en gel de campo pulsante del ADN tratado con la enzima de restriccion SmaI se clasificaron mediante el criterio de Tenover y se analizaron con el programa Fingerprinting II para determinar su relacion genetica con el clon colombiano. Resultados. Todos los aislamientos tuvieron una similitud genetica de 78,5% o mayor con el clon colombiano. Se identificaron 13 subtipos electroforeticos derivados del patron A y cinco de ellos (A5, A6, A8, A13 y A27) constituyeron 61,4% de los aislamientos. Conclusiones. El clon Colombia5 ST289 esta diseminado por America Latina. Esto es importante ya que S. pneumoniae serotipo 5 es causa frecuente de enfermedades invasoras en la Region y esta asociado con la resistencia a trimetoprim-sulfametoxazol. Palabras clave Streptococcus pneumoniae, vigilancia, epidemiologia molecular, electroforesis en gel de campo pulsado, America Latina. Objective. To determine genetic relatedness of clone [Colombia.sup.5] ST289 with invasive Streptococcus pneumoniae serotype 5 isolates recovered in nine Latin American countries. Methods. Forty-four invasive S. pneumoniae serotype 5 isolates recovered from children under 5 years of age in Bolivia, Chile, Dominican Republic, Ecuador, Nicaragua, Panama, Paraguay, Peru, and Venezuela were studied. Pulsed-field gel electrophoresis patterns of DNA treated with SmaI restriction enzyme were classified using Tenover's criteria and analyzed with the Fingerprinting II program to determine their genetic relatedness with the Colombian clone. Results. All isolates had a genetic similarity of 78.5% or more with the Colombian clone. Thirteen electrophoretic subtypes derived of pattern A were identified, and five of them (A5, A6, A8, A13, A27) comprised 61.4% of the isolates. Conclusions. Clone [Colombia.sup.5] ST289 is disseminated in Latin America. This is important because S. pneumoniae serotype 5 frequently causes invasive disease in the region and is associated with trimethoprim-sulfamethoxazole resistance. Key words Streptococcus pneumoniae; epidemiology, molecular; Latin America; electrophoresis, gel, pulsed-field; surveillance, Latin America.