Evaluation of S- and M-Proteins Expressed in IEscherichia coli/I and HEK Cells for Serological Detection of Antibodies in Response to SARS-CoV-2 Infections and mRNA-Based Vaccinations

• Published in: Pathogens (Basel) Vol. 11; no. 12
• Main Authors: Schwarze, Mandy, Luo, Ji, Brakel, Alexandra, Krizsan, Andor, Lakowa, Nicole, Grünewald, Thomas, Lehmann, Claudia, Wolf, Johannes, Borte, Stephan, Milkovska-Stamenova, Sanja, Gabert, Jörg, Scholz, Markus, Hoffmann, Ralf
• Format: Journal Article
• Language: English
• Published: MDPI AG 01.12.2022
• Subjects: Bacterial proteins; Enzyme-linked immunosorbent assay; Escherichia coli; Immune response; Immune system; Immunological research; Physiological aspects; Testing; Germany

This study investigated the IgG and IgA antibody response against recombinant S1 and receptor binding domains (RBD) of the spike (S-) protein and the membrane (M-) protein using a set of 115 serum samples collected from patients infected with SARS-CoV-2 in Germany before April 2021 using protein and...