Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker
Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease‐resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional b...
Saved in:
| Published in | Plant biotechnology journal Vol. 15; no. 7; pp. 817 - 823 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
John Wiley & Sons, Inc
01.07.2017
John Wiley and Sons Inc |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease‐resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR/Cas9/sgRNA technology to modify the canker susceptibility gene CsLOB1 in Duncan grapefruit. Six independent lines, DLOB2, DLOB3, DLOB9, DLOB10, DLOB11 and DLOB12, were generated. Targeted next‐generation sequencing of the six lines showed the mutation rate was 31.58%, 23.80%, 89.36%, 88.79%, 46.91% and 51.12% for DLOB2, DLOB3, DLOB9, DLOB10, DLOB11 and DLOB12, respectively, of the cells in each line. DLOB2 and DLOB3 showed canker symptoms similar to wild‐type grapefruit, when inoculated with the pathogen Xanthomonas citri subsp. citri (Xcc). No canker symptoms were observed on DLOB9, DLOB10, DLOB11 and DLOB12 at 4 days postinoculation (DPI) with Xcc. Pustules caused by Xcc were observed on DLOB9, DLOB10, DLOB11 and DLOB12 in later stages, which were much reduced compared to that on wild‐type grapefruit. The pustules on DLOB9 and DLOB10 did not develop into typical canker symptoms. No side effects and off‐target mutations were detected in the mutated plants. This study indicates that genome editing using CRISPR technology will provide a promising pathway to generate disease‐resistant citrus varieties. |
|---|---|
| AbstractList | Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease‐resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR/Cas9/sgRNA technology to modify the canker susceptibility gene CsLOB1 in Duncan grapefruit. Six independent lines, DLOB2, DLOB3, DLOB9, DLOB10, DLOB11 and DLOB12, were generated. Targeted next‐generation sequencing of the six lines showed the mutation rate was 31.58%, 23.80%, 89.36%, 88.79%, 46.91% and 51.12% for DLOB2, DLOB3, DLOB9, DLOB10, DLOB11 and DLOB12, respectively, of the cells in each line. DLOB2 and DLOB3 showed canker symptoms similar to wild‐type grapefruit, when inoculated with the pathogen Xanthomonas citri subsp. citri (Xcc). No canker symptoms were observed on DLOB9, DLOB10, DLOB11 and DLOB12 at 4 days postinoculation (DPI) with Xcc. Pustules caused by Xcc were observed on DLOB9, DLOB10, DLOB11 and DLOB12 in later stages, which were much reduced compared to that on wild‐type grapefruit. The pustules on DLOB9 and DLOB10 did not develop into typical canker symptoms. No side effects and off‐target mutations were detected in the mutated plants. This study indicates that genome editing using CRISPR technology will provide a promising pathway to generate disease‐resistant citrus varieties. Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease-resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR/Cas9/sgRNA technology to modify the canker susceptibility gene CsLOB1 in Duncan grapefruit. Six independent lines, D[sub.LOB]2, D[sub.LOB]3, D[sub.LOB]9, D[sub.LOB]10, D[sub.LOB]11 and D[sub.LOB]12, were generated. Targeted next-generation sequencing of the six lines showed the mutation rate was 31.58%, 23.80%, 89.36%, 88.79%, 46.91% and 51.12% for D[sub.LOB]2, D[sub.LOB]3, D[sub.LOB]9, D[sub.LOB]10, D[sub.LOB]11 and D[sub.LOB]12, respectively, of the cells in each line. D[sub.LOB]2 and D[sub.LOB]3 showed canker symptoms similar to wild-type grapefruit, when inoculated with the pathogen Xanthomonas citri subsp. citri (Xcc). No canker symptoms were observed on D[sub.LOB]9, D[sub.LOB]10, D[sub.LOB]11 and D[sub.LOB]12 at 4 days postinoculation (DPI) with Xcc. Pustules caused by Xcc were observed on D[sub.LOB]9, D[sub.LOB]10, D[sub.LOB]11 and D[sub.LOB]12 in later stages, which were much reduced compared to that on wild-type grapefruit. The pustules on D[sub.LOB]9 and D[sub.LOB]10 did not develop into typical canker symptoms. No side effects and off-target mutations were detected in the mutated plants. This study indicates that genome editing using CRISPR technology will provide a promising pathway to generate disease-resistant citrus varieties. Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease‐resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR/Cas9/sgRNA technology to modify the canker susceptibility gene CsLOB1 in Duncan grapefruit. Six independent lines, DLOB2, DLOB3, DLOB9, DLOB10, DLOB11 and DLOB12, were generated. Targeted next‐generation sequencing of the six lines showed the mutation rate was 31.58%, 23.80%, 89.36%, 88.79%, 46.91% and 51.12% for DLOB2, DLOB3, DLOB9, DLOB10, DLOB11 and DLOB12, respectively, of the cells in each line. DLOB2 and DLOB3 showed canker symptoms similar to wild‐type grapefruit, when inoculated with the pathogen Xanthomonas citri subsp. citri (Xcc). No canker symptoms were observed on DLOB9, DLOB10, DLOB11 and DLOB12 at 4 days postinoculation (DPI) with Xcc. Pustules caused by Xcc were observed on DLOB9, DLOB10, DLOB11 and DLOB12 in later stages, which were much reduced compared to that on wild‐type grapefruit. The pustules on DLOB9 and DLOB10 did not develop into typical canker symptoms. No side effects and off‐target mutations were detected in the mutated plants. This study indicates that genome editing using CRISPR technology will provide a promising pathway to generate disease‐resistant citrus varieties. Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease-resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR/Cas9/sgRNA technology to modify the canker susceptibility gene CsLOB1 in Duncan grapefruit. Six independent lines, D 2, D 3, D 9, D 10, D 11 and D 12, were generated. Targeted next-generation sequencing of the six lines showed the mutation rate was 31.58%, 23.80%, 89.36%, 88.79%, 46.91% and 51.12% for D 2, D 3, D 9, D 10, D 11 and D 12, respectively, of the cells in each line. D 2 and D 3 showed canker symptoms similar to wild-type grapefruit, when inoculated with the pathogen Xanthomonas citri subsp. citri (Xcc). No canker symptoms were observed on D 9, D 10, D 11 and D 12 at 4 days postinoculation (DPI) with Xcc. Pustules caused by Xcc were observed on D 9, D 10, D 11 and D 12 in later stages, which were much reduced compared to that on wild-type grapefruit. The pustules on D 9 and D 10 did not develop into typical canker symptoms. No side effects and off-target mutations were detected in the mutated plants. This study indicates that genome editing using CRISPR technology will provide a promising pathway to generate disease-resistant citrus varieties. Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing ( HLB ). Breeding for disease‐resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR /Cas9/sg RNA technology to modify the canker susceptibility gene Cs LOB 1 in Duncan grapefruit. Six independent lines, D LOB 2, D LOB 3, D LOB 9, D LOB 10, D LOB 11 and D LOB 12, were generated. Targeted next‐generation sequencing of the six lines showed the mutation rate was 31.58%, 23.80%, 89.36%, 88.79%, 46.91% and 51.12% for D LOB 2, D LOB 3, D LOB 9, D LOB 10, D LOB 11 and D LOB 12, respectively, of the cells in each line. D LOB 2 and D LOB 3 showed canker symptoms similar to wild‐type grapefruit, when inoculated with the pathogen Xanthomonas citri subsp. citri (Xcc). No canker symptoms were observed on D LOB 9, D LOB 10, D LOB 11 and D LOB 12 at 4 days postinoculation ( DPI ) with Xcc. Pustules caused by Xcc were observed on D LOB 9, D LOB 10, D LOB 11 and D LOB 12 in later stages, which were much reduced compared to that on wild‐type grapefruit. The pustules on D LOB 9 and D LOB 10 did not develop into typical canker symptoms. No side effects and off‐target mutations were detected in the mutated plants. This study indicates that genome editing using CRISPR technology will provide a promising pathway to generate disease‐resistant citrus varieties. Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease-resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR/Cas9/sgRNA technology to modify the canker susceptibility gene CsLOB1 in Duncan grapefruit. Six independent lines, DLOB 2, DLOB 3, DLOB 9, DLOB 10, DLOB 11 and DLOB 12, were generated. Targeted next-generation sequencing of the six lines showed the mutation rate was 31.58%, 23.80%, 89.36%, 88.79%, 46.91% and 51.12% for DLOB 2, DLOB 3, DLOB 9, DLOB 10, DLOB 11 and DLOB 12, respectively, of the cells in each line. DLOB 2 and DLOB 3 showed canker symptoms similar to wild-type grapefruit, when inoculated with the pathogen Xanthomonas citri subsp. citri (Xcc). No canker symptoms were observed on DLOB 9, DLOB 10, DLOB 11 and DLOB 12 at 4 days postinoculation (DPI) with Xcc. Pustules caused by Xcc were observed on DLOB 9, DLOB 10, DLOB 11 and DLOB 12 in later stages, which were much reduced compared to that on wild-type grapefruit. The pustules on DLOB 9 and DLOB 10 did not develop into typical canker symptoms. No side effects and off-target mutations were detected in the mutated plants. This study indicates that genome editing using CRISPR technology will provide a promising pathway to generate disease-resistant citrus varieties.Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease-resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance. Here, we used CRISPR/Cas9/sgRNA technology to modify the canker susceptibility gene CsLOB1 in Duncan grapefruit. Six independent lines, DLOB 2, DLOB 3, DLOB 9, DLOB 10, DLOB 11 and DLOB 12, were generated. Targeted next-generation sequencing of the six lines showed the mutation rate was 31.58%, 23.80%, 89.36%, 88.79%, 46.91% and 51.12% for DLOB 2, DLOB 3, DLOB 9, DLOB 10, DLOB 11 and DLOB 12, respectively, of the cells in each line. DLOB 2 and DLOB 3 showed canker symptoms similar to wild-type grapefruit, when inoculated with the pathogen Xanthomonas citri subsp. citri (Xcc). No canker symptoms were observed on DLOB 9, DLOB 10, DLOB 11 and DLOB 12 at 4 days postinoculation (DPI) with Xcc. Pustules caused by Xcc were observed on DLOB 9, DLOB 10, DLOB 11 and DLOB 12 in later stages, which were much reduced compared to that on wild-type grapefruit. The pustules on DLOB 9 and DLOB 10 did not develop into typical canker symptoms. No side effects and off-target mutations were detected in the mutated plants. This study indicates that genome editing using CRISPR technology will provide a promising pathway to generate disease-resistant citrus varieties. |
| Audience | Academic |
| Author | Zhang, Yunzeng Jia, Hongge Orbović, Vladimir Jones, Jeffrey B. Xu, Jin Wang, Nian White, Frank F. |
| AuthorAffiliation | 1 Citrus Research and Education Center Department of Microbiology and Cell Science Institute of Food and Agricultural Sciences (IFAS) University of Florida Lake Alfred FL USA 3 Department of Plant Pathology IFAS University of Florida Gainesville FL USA 2 Citrus Research and Education Center IFAS University of Florida Lake Alfred FL USA |
| AuthorAffiliation_xml | – name: 2 Citrus Research and Education Center IFAS University of Florida Lake Alfred FL USA – name: 3 Department of Plant Pathology IFAS University of Florida Gainesville FL USA – name: 1 Citrus Research and Education Center Department of Microbiology and Cell Science Institute of Food and Agricultural Sciences (IFAS) University of Florida Lake Alfred FL USA |
| Author_xml | – sequence: 1 givenname: Hongge surname: Jia fullname: Jia, Hongge organization: University of Florida – sequence: 2 givenname: Yunzeng surname: Zhang fullname: Zhang, Yunzeng organization: University of Florida – sequence: 3 givenname: Vladimir surname: Orbović fullname: Orbović, Vladimir organization: University of Florida – sequence: 4 givenname: Jin surname: Xu fullname: Xu, Jin organization: University of Florida – sequence: 5 givenname: Frank F. surname: White fullname: White, Frank F. organization: University of Florida – sequence: 6 givenname: Jeffrey B. surname: Jones fullname: Jones, Jeffrey B. organization: University of Florida – sequence: 7 givenname: Nian surname: Wang fullname: Wang, Nian email: nianwang@ufl.edu organization: University of Florida |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27936512$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkktv3CAUhVGVKu9F_0CF1E03MzFgLnhTKRm1aaSR0kWzRhhfO6Q2TI2dav59yaPTpqpUWHDF_e4ROpwjshdiQELesGLJ8jrb1H7JOCj1ihyyEtRCgeR7u7osD8hRSndFwRlI2CcHXFUCJOOHpL3EEAek2PjJh47Glk63SBuf0CakaU4ON5Ovfe-nLe0wIF2l9fUFoz5Q56dxTtTF0OKY6IjJp8kGh3SKu6YN33A8Ia9b2yc8fT6Pyc2nj19Xnxfr68ur1fl60UldqQVoFC3ThS6cqFRbO0RXttIha4SsQADUpaiFUE6DRNtICU0DljUcQAutxDH58KS7mesBG4dhGm1vNqMf7Lg10XrzshP8renivZElQCkgC7x_Fhjj9xnTZAafLeh7GzDOyfBCCl1xXvwfZVpykJVkZUbf_YXexXkM2QnDqkJJqQXXv6nO9mh8aGN-onsQNedKCF4KxUWmlv-g8m5w8PkrsPX5_sXA2z892ZnxKwQZOHsCfuTJ7a7PCvOQLpPTZR7TZb5cXD0W4icW8cHm |
| ContentType | Journal Article |
| Copyright | 2016 The Authors. published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. COPYRIGHT 2017 John Wiley & Sons, Inc. 2017. This work is published under https://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: 2016 The Authors. published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. – notice: 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. – notice: COPYRIGHT 2017 John Wiley & Sons, Inc. – notice: 2017. This work is published under https://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | 24P CGR CUY CVF ECM EIF NPM 7QO 8FD 8FE 8FG 8FH ABJCF ABUWG AEUYN AFKRA AZQEC BBNVY BENPR BGLVJ BHPHI CCPQU DWQXO FR3 GNUQQ HCIFZ L6V LK8 M7P M7S P64 PHGZM PHGZT PIMPY PKEHL PQEST PQGLB PQQKQ PQUKI PRINS PTHSS 7X8 7S9 L.6 5PM |
| DOI | 10.1111/pbi.12677 |
| DatabaseName | Wiley Online Library Open Access Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Biotechnology Research Abstracts Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection ProQuest One Community College ProQuest Central Korea Engineering Research Database ProQuest Central Student SciTech Premium Collection ProQuest Engineering Collection Biological Sciences Biological Science Database Engineering Database Biotechnology and BioEngineering Abstracts ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection MEDLINE - Academic AGRICOLA AGRICOLA - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Publicly Available Content Database ProQuest Central Student Technology Collection Technology Research Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Central China ProQuest Central ProQuest One Applied & Life Sciences ProQuest One Sustainability ProQuest Engineering Collection Biotechnology Research Abstracts Natural Science Collection ProQuest Central Korea Biological Science Collection ProQuest Central (New) Engineering Collection Engineering Database ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Technology Collection Biological Science Database ProQuest SciTech Collection Biotechnology and BioEngineering Abstracts ProQuest One Academic UKI Edition Materials Science & Engineering Collection Engineering Research Database ProQuest One Academic ProQuest One Academic (New) MEDLINE - Academic AGRICOLA AGRICOLA - Academic |
| DatabaseTitleList | Publicly Available Content Database MEDLINE AGRICOLA MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: 24P name: Wiley Online Library Open Access url: https://authorservices.wiley.com/open-science/open-access/browse-journals.html sourceTypes: Publisher – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Agriculture |
| DocumentTitleAlternate | Hongge Jia et al |
| EISSN | 1467-7652 |
| EndPage | 823 |
| ExternalDocumentID | PMC5466436 A733243723 27936512 PBI12677 |
| Genre | article Journal Article |
| GrantInformation_xml | – fundername: Citrus Research and Development Foundation |
| GroupedDBID | --- .3N .GA .Y3 05W 0R~ 10A 123 1OC 24P 29O 31~ 33P 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52W 52X 53G 5HH 5LA 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8FE 8FG 8FH 8UM 930 A03 A8Z AAEVG AAHBH AAHHS AANHP AAONW AAZKR ABCQN ABDBF ABEML ABIJN ABJCF ABPVW ACBWZ ACCFJ ACCMX ACIWK ACPRK ACRPL ACSCC ACUHS ACXQS ACYXJ ADBBV ADIZJ ADKYN ADNMO ADZMN AEEZP AEIMD AENEX AEQDE AEUQT AEUYN AFBPY AFEBI AFKRA AFRAH AFZJQ AIWBW AJBDE ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR ASPBG ATUGU AVUZU AVWKF AZBYB AZFZN BAFTC BBNVY BCNDV BDRZF BENPR BFHJK BGLVJ BHPHI BNHUX BROTX BRXPI BY8 CAG CCPQU COF CS3 D-E D-F DPXWK DR2 DU5 EAD EAP EBD EBS ECGQY EDH EJD EMK EMOBN EST ESX F00 F01 F04 F5P FEDTE G-S G.N GODZA GROUPED_DOAJ H.T H.X HCIFZ HF~ HOLLA HVGLF HZ~ IAO IEP IGS IHE ITC IX1 J0M KQ8 L6V LC2 LC3 LH4 LK8 LP6 LP7 LW6 M7P M7S MK4 ML0 N04 N05 N9A NF~ O66 O9- OIG OK1 P2P P2X P4D PIMPY PROAC PTHSS Q.N Q11 QB0 QM4 QO4 R.K ROL RPM RX1 SUPJJ SV3 TUS UB1 W8V W99 WIH WIN WQJ WRC XG1 ~IA ~KM ~WT CGR CUY CVF ECM EIF NPM PHGZM PHGZT PMFND 7QO 8FD AAMMB ABUWG AEFGJ AGXDD AIDQK AIDYY AZQEC DWQXO FR3 GNUQQ P64 PKEHL PQEST PQGLB PQQKQ PQUKI PRINS 7X8 7S9 L.6 5PM |
| ID | FETCH-LOGICAL-g5897-68e3f18080c397fbceec4f5ce1d3596366b43b337c865ead556dd6a1d26683873 |
| IEDL.DBID | DR2 |
| ISSN | 1467-7644 1467-7652 |
| IngestDate | Thu Aug 21 18:31:40 EDT 2025 Fri Jul 11 18:24:05 EDT 2025 Fri Jul 11 06:23:22 EDT 2025 Wed Aug 13 03:05:16 EDT 2025 Tue Jun 17 21:30:10 EDT 2025 Tue Jun 10 20:15:51 EDT 2025 Wed Feb 19 02:41:56 EST 2025 Wed Jan 22 16:19:52 EST 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 7 |
| Keywords | Xanthomonas citri Cas9 sgRNA Citrus paradisi |
| Language | English |
| License | Attribution 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-g5897-68e3f18080c397fbceec4f5ce1d3596366b43b337c865ead556dd6a1d26683873 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | https://proxy.k.utb.cz/login?url=https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fpbi.12677 |
| PMID | 27936512 |
| PQID | 1907558328 |
| PQPubID | 1096352 |
| PageCount | 7 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_5466436 proquest_miscellaneous_2053892206 proquest_miscellaneous_1852659514 proquest_journals_1907558328 gale_infotracmisc_A733243723 gale_infotracacademiconefile_A733243723 pubmed_primary_27936512 wiley_primary_10_1111_pbi_12677_PBI12677 |
| PublicationCentury | 2000 |
| PublicationDate | July 2017 |
| PublicationDateYYYYMMDD | 2017-07-01 |
| PublicationDate_xml | – month: 07 year: 2017 text: July 2017 |
| PublicationDecade | 2010 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England – name: Southampton – name: Hoboken |
| PublicationTitle | Plant biotechnology journal |
| PublicationTitleAlternate | Plant Biotechnol J |
| PublicationYear | 2017 |
| Publisher | John Wiley & Sons, Inc John Wiley and Sons Inc |
| Publisher_xml | – name: John Wiley & Sons, Inc – name: John Wiley and Sons Inc |
| References | 2010; 22 2010; 26 2011; 1 2015; 82 2013; 84 2015; 10 2013; 30 2016b; 532 2011; 11 2015; 1224 2016 2014; 111 2012; 337 2016a; 34 2007; 35 2016; 14 2005; 23 2014; 346 2014; 33 2012; 30 2014; 32 25146436 - Plant Cell Rep. 2014 Dec;33(12):1993-2001 20709691 - Bioinformatics. 2010 Oct 1;26(19):2460-1 27111611 - Nature. 2016 Apr 21;532(7599):293 27071672 - Plant Biotechnol J. 2016 May;14 (5):1291-301 15694124 - Biotechnol Adv. 2005 Mar;23(2):131-71 25430774 - Science. 2014 Nov 28;346(6213):1258096 24474801 - Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):E521-9 17913740 - Nucleic Acids Res. 2007;35(19):6663-71 27539813 - Plant Biotechnol J. 2017 Mar;15(3):306-317 21439092 - BMC Plant Biol. 2011 Mar 28;11:55 21097711 - Plant Cell. 2010 Nov;22(11):3662-77 25004231 - Nat Biotechnol. 2014 Jul;32(7):640-2 27276658 - Mol Plant Pathol. 2016 Jun 8;:null 25416263 - Methods Mol Biol. 2015;1224:245-57 24132122 - Mol Biol Evol. 2013 Dec;30(12):2725-9 27281401 - Nat Biotechnol. 2016 Jun 9;34(6):582 26398891 - PLoS One. 2015 Sep 23;10(9):e0137134 22565958 - Nat Biotechnol. 2012 May 07;30(5):390-2 22745249 - Science. 2012 Aug 17;337(6096):816-21 25824104 - Plant J. 2015 May;82(4):632-43 22384362 - G3 (Bethesda). 2011 Nov;1(6):515-22 |
| References_xml | – volume: 1 start-page: 515 year: 2011 end-page: 522 article-title: Genome‐scale analysis of programmed DNA elimination sites in publication-title: G3: Genes – Genomes – Genet. – volume: 1224 start-page: 245 year: 2015 end-page: 257 article-title: Citrus transformation using juvenile tissue explants publication-title: Methods Mol. Biol. – year: 2016 article-title: Targeted promoter editing for rice resistance to pv. oryzae reveals differential activities for ‐inducing TAL effectors publication-title: Plant Biotechnol – volume: 532 start-page: 293 year: 2016b article-title: Gene‐edited CRISPR mushroom escapes US regulation publication-title: Nature – volume: 111 start-page: E521 year: 2014 end-page: E529 article-title: Lateral organ boundaries 1 is a disease susceptibility gene for citrus bacterial canker disease publication-title: Proc. Natl. Acad. Sci. USA – volume: 32 start-page: 640 year: 2014 end-page: 642 article-title: A genealogy of the citrus family publication-title: Nat. Biotechnol. – volume: 10 start-page: e0137134 year: 2015 article-title: Transgenic citrus expressing an Arabidopsis gene exhibit enhanced resistance against Huanglongbing (HLB; Citrus Greening) publication-title: PLoS ONE – volume: 26 start-page: 2460 year: 2010 end-page: 2461 article-title: Search and clustering orders of magnitude faster than BLAST publication-title: Bioinformatics – volume: 11 start-page: 55 year: 2011 article-title: Ectopic expression of in sweet orange ( Osbeck) reduces canker susceptibility: involvement of H₂O₂ production and transcriptional alteration publication-title: BMC Plant Biol. – volume: 23 start-page: 131 year: 2005 end-page: 171 article-title: Plant protoplasts: status and biotechnological perspectives publication-title: Biotechnol. Adv. – year: 2016 article-title: Homologs of in citrus function as disease susceptibility genes in citrus canker publication-title: Mol. Plant Pathol. – volume: 30 start-page: 2725 year: 2013 end-page: 2729 article-title: MEGA6: molecular evolutionary genetics analysis version 6.0 publication-title: Mol. Biol. Evol. – volume: 22 start-page: 3662 year: 2010 end-page: 3677 article-title: Members of the LATERAL ORGAN BOUNDARIES DOMAIN transcription factor family are involved in the regulation of secondary growth in Populus publication-title: Plant Cell – volume: 33 start-page: 1993 year: 2014 end-page: 2001 article-title: Xcc‐facilitated agroinfiltration of citrus leaves: a tool for rapid functional analysis of transgenes in citrus leaves publication-title: Plant Cell Rep. – volume: 82 start-page: 632 year: 2015 end-page: 643 article-title: Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice publication-title: Plant J. – volume: 35 start-page: 6663 year: 2007 end-page: 6671 article-title: LATERAL ORGAN BOUNDARIES defines a new family of DNA‐binding transcription factors and can interact with specific bHLH proteins publication-title: Nucleic Acids Res. – volume: 30 start-page: 390 year: 2012 end-page: 392 article-title: High‐efficiency TALEN‐based gene editing produces disease‐resistant rice publication-title: Nat. Biotechnol. – volume: 346 start-page: 1258096 year: 2014 article-title: Genome editing. The new frontier of genome engineering with CRISPR‐Cas9 publication-title: Science – volume: 14 start-page: 1291 year: 2016 end-page: 1301 article-title: Modification of the PthA4 effector binding elements in Type I promoter using Cas9/sgRNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCsLOB1.3 infection publication-title: Plant Biotechnol. J. – volume: 34 start-page: 582 year: 2016a article-title: CRISPR‐edited crops free to enter market, skip regulation publication-title: Nat. Biotechnol. – volume: 337 start-page: 816 year: 2012 end-page: 821 article-title: A programmable dual‐RNA‐guided DNA endonuclease in adaptive bacterial immunity publication-title: Science – volume: 84 start-page: 115 year: 2013 end-page: 122 article-title: Over‐expression of the citrus gene confers resistance to bacterial canker disease publication-title: Physiol. Mol. Plant Pathol. – reference: 26398891 - PLoS One. 2015 Sep 23;10(9):e0137134 – reference: 27111611 - Nature. 2016 Apr 21;532(7599):293 – reference: 25430774 - Science. 2014 Nov 28;346(6213):1258096 – reference: 22565958 - Nat Biotechnol. 2012 May 07;30(5):390-2 – reference: 27281401 - Nat Biotechnol. 2016 Jun 9;34(6):582 – reference: 24474801 - Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):E521-9 – reference: 25824104 - Plant J. 2015 May;82(4):632-43 – reference: 25146436 - Plant Cell Rep. 2014 Dec;33(12):1993-2001 – reference: 17913740 - Nucleic Acids Res. 2007;35(19):6663-71 – reference: 15694124 - Biotechnol Adv. 2005 Mar;23(2):131-71 – reference: 20709691 - Bioinformatics. 2010 Oct 1;26(19):2460-1 – reference: 27071672 - Plant Biotechnol J. 2016 May;14 (5):1291-301 – reference: 27276658 - Mol Plant Pathol. 2016 Jun 8;:null – reference: 21097711 - Plant Cell. 2010 Nov;22(11):3662-77 – reference: 22384362 - G3 (Bethesda). 2011 Nov;1(6):515-22 – reference: 21439092 - BMC Plant Biol. 2011 Mar 28;11:55 – reference: 27539813 - Plant Biotechnol J. 2017 Mar;15(3):306-317 – reference: 25416263 - Methods Mol Biol. 2015;1224:245-57 – reference: 24132122 - Mol Biol Evol. 2013 Dec;30(12):2725-9 – reference: 25004231 - Nat Biotechnol. 2014 Jul;32(7):640-2 – reference: 22745249 - Science. 2012 Aug 17;337(6096):816-21 |
| SSID | ssj0021656 |
| Score | 2.6294792 |
| Snippet | Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and... |
| SourceID | pubmedcentral proquest gale pubmed wiley |
| SourceType | Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 817 |
| SubjectTerms | adverse effects Bacteria bacterial canker breeding Candidatus Liberibacter asiaticus Canker Cas9 Citrus - genetics Citrus - microbiology Citrus canker Citrus fruits Citrus greening Citrus paradisi CRISPR crop production Disease control Disease resistance Disease Resistance - genetics Disease susceptibility Disease Susceptibility - metabolism Editing Fruits Gene Editing Gene Expression Regulation, Plant - genetics genes Genetic aspects Genetic research Genome editing Genomes Genomics Grapefruit grapefruits greening disease Health aspects high-throughput nucleotide sequencing juvenility Mutation Pathogens Plant bacterial diseases Plant breeding Plant diseases Plant Diseases - genetics Plant Diseases - microbiology Plants (botany) Plants, Genetically Modified - genetics Plants, Genetically Modified - microbiology polyembryony Polyploidy RNA, Plant - genetics sgRNA Side effects Target detection Tree crops trees Xanthomonas citri |
| SummonAdditionalLinks | – databaseName: ProQuest Central dbid: BENPR link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3LbtQwFLVguoFFxbuBgoyEBBvDxO-s0EzVUhCUClGpuyh27DJCTYamXfD33OvxhA4IVolkO1Jyj-_DOT4m5AXnlY9uGlgpo2NSNo65KgjmnYfr1JvK4XrHpyN9eCI_nKrTvOA2ZFrl2icmR932HtfI30DgMkoB_uzb5Q-Gp0bh39V8hMZNsgUu2NoJ2ZrvHx1_GUsu1JZZ7S8yzEDoz9pCyOVZusXrkmtj_nbG16LRn0zJ6xlsCkEHd8h2zh3pbGXsu-RG6O6R27Ozi6yfEe6T-C50_XmgEJKQz0z7SCHDo_k3DB2uhkRjSYzYnxTAE-je8PHzvKSLjvq0A4P6tAlwoFCIY3IJqKCX_djYdN_DxQNycrD_de-Q5aMU2JmylWHaBhFL1JD0kIBEB6HRy6h8KFuhYA5q7aRwQhhvtQJwKaXbVjdlC_HbCmvEQzLp-i7sEFoJEadViKWJULu0wRkoMZs2yih1EJUsyEv8nDVOEPhmvsk8fxiNUlP1zAiBKohcFGR3oycA2282rw1S54k11L9hUJDnYzOORLJYF_or6GNR8x9SR_nvPhycj604n-qCPFrZuF6u9D1qDj5LQyJUELNh_bEDSnJvtnSLb0maW6Fav4Bnvko4GUesSy0AXZ1AVx_P36ebx_9_yyfkFsdEIhGEd8kETB2eQhp06Z5lrP8C6-kIZQ priority: 102 providerName: ProQuest |
| Title | Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fpbi.12677 https://www.ncbi.nlm.nih.gov/pubmed/27936512 https://www.proquest.com/docview/1907558328 https://www.proquest.com/docview/1852659514 https://www.proquest.com/docview/2053892206 https://pubmed.ncbi.nlm.nih.gov/PMC5466436 |
| Volume | 15 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ba9swFD503cv2sPvFXRc0GGwvDrEkSzZ7SkrTbmxdKCv0YWAsWepCqVPq5GH79TtHdkzSMRh7SQI6Cg46l0_Kdz4BvOU8t96MXJxIb2IpSxOb3InYGovvI6tzQ-cdX07U8Zn8dJ6e78CHdS9Mqw_RH7hRZIR8TQFemmYjyK_NfJhwpamTnLhaBIhOe-koTqoybWeRjjUW_U5ViFg8_cw_0_BGHbrNkdzErqH4TB_C9_Vjt5yTy-FqaYb21y1Fx__8XY_gQQdK2bj1osew4-oncH98cdMJc7in4I9cvbhyDGsdEaXZwjOEjqz7f4c1qybwYwLV9idDr3TsoPn8dZKwec1saO1gNnQXNgx3-IRa0d3YctEPlvWlu3kGZ9PDbwfHcXdHQ3yRZrmOVeaET0ic0iKy8QZrrpU-tS6pRIrBrZSRwgihbaZS9No0VVWlyqRCYJCJTIvnsFsvavcSWC6EH-XOJ9rjpqhyRuPetay89FI5kcsI3tFqFRR5uCS27BoIcDZpWBVjLQTJK3IRwf6WJUaM3R5er3fRRWxTIDDSaYr5LYvgTT9MM4mFVrvFCm0yukwAMan8uw3HrJblnI9UBC9aFyquW-GQgmMyVIiwItBbztUbkNb39kg9_xE0v1O6BkDgd74PvtPPWO_h0GuK4DXFbPIxfNj7d9NXcI8TWgks5H3YxWV3rxFrLc0A7nA5w9dsejSAu5PDk9npIJxbDEK4_QZmwitW |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwELZKOQAHxJtAASOB4BLY-JkcENoWtrt0Wzi0Um8hduyyQiRL0wr1T_EbmXEedEFw6ymRbEeK5_PMN8k8CHnGWGa9Gbk4Ed7EQhQmNpnjsTUWriOrM4PfO3b31PRAfDiUh2vkZ58Lg2GVvU4MirqsLX4jfw2GS0sJ-EvfLr_H2DUK_672LTRaWOy4sx_gsjVvZu9Avs8Zm7zf35rGXVeB-EimmY5V6rhPsJyiBVvsDVgJK7y0Lim5BDgqZQQ3nGubKgn7LKUqS1UkJZiylKeaw3MvkcuCw2rMTJ9sDw4eVrJps5l0rIFodJWMMHJoaRavEqa0_lv1n7N9f8ZlnufLweBNbpDrHVOl4xZaN8maq26Ra-Oj465ah7tN_Lar6m-OggHE6Glaewp8knY_fWhz2oSgmRB_e0YBqo5uNfOPmwldVNSGfA9qQ8phQ8HtRyoLGKQn9TBYVF_d8R1ycCFbfJesV3Xl7hOace5HmfOJ9uAplc5ocGiL0gsvlOOZiMgL3M4cjyPsmS26rAJYjYWt8rHmHGsuMh6RjZWZcIzs6nAvkLw7xk3-G3QReToM40oMTatcfQpzUuwwAERV_HsOA1WXZoyNVETutTLOl201kZyBhlRAuyKiV6Q_TMAC4Ksj1eJLKAQusTcAh2e-DDgZVvSOHYAuD6DLP23Ows2D_7_lE3Jlur87z-ezvZ2H5CpDChNCkzfIOojdPQICdmIeB9RT8vmij9kv2gtDJQ |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db9MwED-NTkLsAfG9wgAjgeAlW2MndvKAULutrGyUCjFpb6F27FEhktJsQvvX-Ou4c5OwguBtT4lkO1J8v_P9LrkPgOecp8bpng3CyOkgiqY60KkVgdEGrz2jUk3fO96P5cFx9O4kPlmDn00uDIVVNmeiP6jz0tA38h00XCqOEX_JjqvDIiZ7wzfz7wF1kKI_rU07jSVEDu3FD3TfqtejPZT1C86H-592D4K6w0BwGiepCmRihQuptKJBu-w0WgwTudjYMBcxQlNKHQkthDKJjHHP41jmuZyGOZq1RCRK4HOvwboir6gD64P98eRj6-5RXZtlbpMKFNKOuq4RxRHN9Ww75FKpvw3BJUv4Z5TmZfbszd_wFtyseSvrL4F2G9ZscQc2-qeLunaHvQvurS3Kb5ahOaRYalY6huyS1b-AWHVe-RAaH417wRC4lu1WRx8GIZsVzPjsD2Z8AmLFFrYiYouIZGdlOzgtvtrFPTi-kk2-D52iLOwmsFQI10utC5VDvym3WqF7O81d5CJpRRp14SVtZ0bKiXtmpnWOAa6mMldZXwlBFRi56MLWykxUKrM63Agkq5W6yn5DsAvP2mFaSYFqhS3PcU5C_QaQtkb_nsPx4EtSznuyCw-WMs7my9oiGcfzUiIJ64JakX47gcqBr44Usy--LHhMnQIEPvOVx0m7onHzEHSZB102GYz8zcP_v-VTuI4qlh2NxoeP4AYnPuPjlLegg1K3j5GNneknNewZfL5qTfsFSH5Itw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Genome+editing+of+the+disease+susceptibility+gene+CsLOB1+in+citrus+confers+resistance+to+citrus+canker&rft.jtitle=Plant+biotechnology+journal&rft.au=Jia%2C+Hongge&rft.au=Zhang%2C+Yunzeng&rft.au=Orbovi%C4%87%2C+Vladimir&rft.au=Xu%2C+Jin&rft.date=2017-07-01&rft.issn=1467-7644&rft.eissn=1467-7652&rft.volume=15&rft.issue=7&rft.spage=817&rft.epage=823&rft_id=info:doi/10.1111%2Fpbi.12677&rft.externalDBID=10.1111%252Fpbi.12677&rft.externalDocID=PBI12677 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1467-7644&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1467-7644&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1467-7644&client=summon |