ARGOS8 variants generated by CRISPR‐Cas9 improve maize grain yield under field drought stress conditions

• Published in: Plant biotechnology journal Vol. 15; no. 2; pp. 207 - 216
• Main Authors: Shi, Jinrui, Gao, Huirong, Wang, Hongyu, Lafitte, H. Renee, Archibald, Rayeann L., Yang, Meizhu, Hakimi, Salim M., Mo, Hua, Habben, Jeffrey E.
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.02.2017; John Wiley and Sons Inc
• Subjects: Agricultural production; alleles; Amino acids; ARGOS; Base Sequence; Biodiversity; breeding; Corn; CRISPR; CRISPR-Associated Proteins - metabolism; CRISPR-Cas Systems; CRISPR‐Cas9; Crop yield; Crop yields; crops; Crops, Agricultural - genetics; Deoxyribonucleic acid; DNA; DNA repair; DNA sequencing; Drought; Drought resistance; drought tolerance; Droughts; Edible Grain - genetics; Ethylene; Ethylenes; Flowering; Gene Editing; Gene expression; Gene Expression Regulation, Plant; Genes; Genetic engineering; Genetically modified plants; genome editing; Genomes; Grain; grain yield; loci; maize; messenger RNA; Plant breeding; Plant Proteins - genetics; Plants, Genetically Modified; Polymerase Chain Reaction; promoter regions; Promoter Regions, Genetic; Proteins; RNA; RNA, Messenger - genetics; sequence analysis; Signal transduction; Stress, Physiological - genetics; Transgenic plants; water stress; Zea mays; Zea mays - genetics; ARGOS; genome editing; drought tolerance; grain yield; maize; CRISPR-Cas9

Summary Maize ARGOS8 is a negative regulator of ethylene responses. A previous study has shown that transgenic plants constitutively overexpressing ARGOS8 have reduced ethylene sensitivity and improved grain yield under drought stress conditions. To explore the targeted use of ARGOS8 native expressi...