Blockade of senescence‐associated microRNA‐195 in aged skeletal muscle cells facilitates reprogramming to produce induced pluripotent stem cells

• Published in: Aging cell Vol. 15; no. 1; pp. 56 - 66
• Main Authors: Kondo, Hideyuki, Kim, Ha Won, Wang, Lei, Okada, Motoi, Paul, Christian, Millard, Ronald W., Wang, Yigang
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.02.2016; John Wiley and Sons Inc
• Subjects: Age Factors; aging cell; Animals; Cell Differentiation - physiology; Cells, Cultured; Cellular Reprogramming - genetics; Cellular Senescence; Comparative analysis; Fibroblasts - cytology; Induced Pluripotent Stem Cells - metabolism; Mice; Mice, Inbred C57BL; MicroRNA; MicroRNAs - metabolism; Muscle Fibers, Skeletal - cytology; Muscle Fibers, Skeletal - metabolism; Muscles; Original; reprogramming; Stem cells; Telomerase; telomere length; Telomeres; reprogramming; microRNA; aging cell; telomere length

Summary The low reprogramming efficiency in cells from elderly patients is a challenge that must be overcome. Recently, it has been reported that senescence‐associated microRNA (miR)‐195 targets Sirtuin 1 (SIRT1) to advance cellular senescence. Thus, we hypothesized that a blockade of miR‐195 expres...