Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1451; p. 207
• Main Authors: Matty, Molly A, Oehlers, Stefan H, Tobin, David M
• Format: Journal Article
• Language: English
• Published: United States 2016
• Subjects: Animals; Host-Pathogen Interactions - genetics; Host-Pathogen Interactions - immunology; Host-Pathogen Interactions - physiology; Immunity, Innate - genetics; Immunity, Innate - physiology; Larva - immunology; Larva - metabolism; Larva - microbiology; Mycobacterium marinum - immunology; Mycobacterium marinum - pathogenicity; Zebrafish - immunology; Zebrafish - metabolism; Zebrafish - microbiology; Infection; Innate immunity; Zebrafish; Time-lapse imaging; Drug treatment; Mycobacteria

Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larv...