Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae

Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larv...

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Published inMethods in molecular biology (Clifton, N.J.) Vol. 1451; p. 207
Main Authors Matty, Molly A, Oehlers, Stefan H, Tobin, David M
Format Journal Article
LanguageEnglish
Published United States 2016
Subjects
Animals
Host-Pathogen Interactions - genetics
Host-Pathogen Interactions - immunology
Host-Pathogen Interactions - physiology
Immunity, Innate - genetics
Immunity, Innate - physiology
Larva - immunology
Larva - metabolism
Larva - microbiology
Mycobacterium marinum - immunology
Mycobacterium marinum - pathogenicity
Zebrafish - immunology
Zebrafish - metabolism
Zebrafish - microbiology
Infection
Innate immunity
Zebrafish
Time-lapse imaging
Drug treatment
Mycobacteria
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Abstract Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.
AbstractList Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.
Author Tobin, David M
Matty, Molly A
Oehlers, Stefan H
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  givenname: Stefan H
  surname: Oehlers
  fullname: Oehlers, Stefan H
  organization: Department of Molecular Genetics and Microbiology, Center for Host-Microbial Interactions, Duke University Medical Center, 219 Jones Building, Box 3020 DUMC, Durham, NC, 27710, USA
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  givenname: David M
  surname: Tobin
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  email: david.tobin@duke.edu, david.tobin@duke.edu
  organization: Department of Immunology, Duke University Medical Center, Durham, NC, 27710, USA. david.tobin@duke.edu
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Keywords Infection
Innate immunity
Zebrafish
Time-lapse imaging
Drug treatment
Mycobacteria
Language English
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PublicationTitle Methods in molecular biology (Clifton, N.J.)
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Snippet Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish...
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StartPage 207
SubjectTerms Animals
Host-Pathogen Interactions - genetics
Host-Pathogen Interactions - immunology
Host-Pathogen Interactions - physiology
Immunity, Innate - genetics
Immunity, Innate - physiology
Larva - immunology
Larva - metabolism
Larva - microbiology
Mycobacterium marinum - immunology
Mycobacterium marinum - pathogenicity
Zebrafish - immunology
Zebrafish - metabolism
Zebrafish - microbiology
Title Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae
URI https://www.ncbi.nlm.nih.gov/pubmed/27464810
Volume 1451
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