Characterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters

• Published in: Biotechnology and bioengineering Vol. 69; no. 5; pp. 486 - 494
• Main Authors: Boer, Harry, Teeri, Tuula T., Koivula, Anu
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 05.09.2000; Wiley
• Subjects: activity screening; Biological and medical sciences; Biotechnology; Biotechnology - methods; Catalyst activity; Cel7A protein; cellobiohydrolase; cellobiose; Cellulase; Cellulase - isolation & purification; Cellulase - metabolism; Cellulase - secretion; cellulolytic microorganisms; Cellulose; Cellulose - metabolism; Cellulose 1,4-beta-Cellobiosidase; enzymology; expression; Fermentation; Fermenters; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Genetic engineering; Genetic technics; Genetic Testing; Genetic Testing - methods; genetics; glyceraldehyde-3-phosphate dehydrogenase; glycosidases; Glycosylation; Hot Temperature; isolation & purification; metabolism; methods; Methods. Procedures. Technologies; Modification of gene expression level; Pichia; Pichia - enzymology; Pichia - genetics; Pichia pastoris; Promoter Regions, Genetic; Promoter Regions, Genetic - genetics; Saccharomyces cerevisiae; secretion; Solubility; stability; Thermodynamic stability; Transformation, Genetic; Trichoderma; Trichoderma - enzymology; Trichoderma - genetics; Trichoderma reesei; Yeast; Yeast; Enzyme; Transcription promoter; Secretion; Glycosylation; Cellulose 1,4-β-cellobiosidase; Gene expression; Trichoderma reesei; Thermal stability; Fungi; Screening; Enzymatic activity; Glycosidases; Hydrolases; Ascomycetes; Fungi Imperfecti; Recombinant protein; Vector; Hybrid gene; Physicochemical properties; Thallophyta

Heterologous expression of T. reesei cellobiohydrolase Cel7A in a methylotrophic yeast Pichia pastoris was tested both under the P. pastoris alcohol oxidase (AOX1) promoter and the glyceraldehyde‐3‐phosphate dehydrogenase (GAP) promoter in a fermentor. Production of Cel7A with the AOX1 promoter gave...