Characterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters - Boer, Harry; Teeri, Tuula T.; Koivula, Anu (Biotechnology and bioengineering, 2000)
Keywords: activity screening; Biological and medical sciences; Biotechnology; Biotechnology - methods; Catalyst activity; Cel7A protein; cellobiohydrolase; cellobiose; Cellulase; Cellulase - isolation & purification; Cellulase - metabolism; Cellulase - secretion; cellulolytic microorganisms; Cellulose; Cellulose - metabolism; Cellulose 1,4-beta-Cellobiosidase; enzymology; expression; Fermentation; Fermenters; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Fungal; Genetic engineering; Genetic technics; Genetic Testing; Genetic Testing - methods; genetics; glyceraldehyde-3-phosphate dehydrogenase; glycosidases; Glycosylation; Hot Temperature; isolation & purification; metabolism; methods; Methods. Procedures. Technologies; Modification of gene expression level; Pichia; Pichia - enzymology; Pichia - genetics; Pichia pastoris; Promoter Regions, Genetic; Promoter Regions, Genetic - genetics; Saccharomyces cerevisiae; secretion; Solubility; stability; Thermodynamic stability; Transformation, Genetic; Trichoderma; Trichoderma - enzymology; Trichoderma - genetics; Trichoderma reesei; Yeast; Yeast; Enzyme; Transcription promoter; Secretion; Glycosylation; Cellulose 1,4-β-cellobiosidase; Gene expression; Trichoderma reesei; Thermal stability; Fungi; Screening; Enzymatic activity; Glycosidases; Hydrolases; Ascomycetes; Fungi Imperfecti; Recombinant protein; Vector; Hybrid gene; Physicochemical properties; Thallophyta