Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from Rhodococcus erythropolis
Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli. Crude cell free extracts were used for the hydrolysis of different aromatic cyanohydrins. Nitrile hydratase expression was increased up to 5-fold by redesign of the expression ca...
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| Published in | Biotechnology letters Vol. 26; no. 21; pp. 1675 - 1680 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Dordrecht
Springer
01.11.2004
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli. Crude cell free extracts were used for the hydrolysis of different aromatic cyanohydrins. Nitrile hydratase expression was increased up to 5-fold by redesign of the expression cassette. The recombinant enzymes were successfully used for the conversion of several cyanohydrins to the corresponding alpha-hydroxy amides and acids while retaining enantiopurity. |
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| AbstractList | Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli. Crude cell free extracts were used for the hydrolysis of different aromatic cyanohydrins. Nitrile hydratase expression was increased up to 5-fold by redesign of the expression cassette. The recombinant enzymes were successfully used for the conversion of several cyanohydrins to the corresponding alpha-hydroxy amides and acids while retaining enantiopurity. Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli.Crude cell free extracts were used for the hydrolysis of different aromatic cyanohydrins. Nitrile hydratase expression was increased up to 5-fold by redesign of the expression cassette. The recombinant enzymes were successfully used for the conversion of several cyanohydrins to the corresponding I plus or minus -hydroxy amides and acids while retaining enantiopurity. |
| Author | GLIEDER, A SCHWAB, H REISINGER, Ch SCHOEMAKER, H. E GRIENGL, H OSPRIAN, I |
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| Keywords | Enzyme Amidase Rhodococcus erythropolis Lyases Hydrolysis cyanohydrin hydrolysis Cyanohydrin Biotransformation Nitrile hydratase Bacteria Hydrolases Actinomycetes Carbon-oxygen lyases Hydro-lyases Enzymatic reaction |
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| Snippet | Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli. Crude cell free extracts were used... Nitrile hydratase and amidase from Rhodococcus erythropolis CIMB11540 were both cloned and expressed in Escherichia coli.Crude cell free extracts were used for... |
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| SubjectTerms | Amidohydrolases - chemistry Amidohydrolases - genetics Amidohydrolases - metabolism Biological and medical sciences Biotechnology Cloning, Molecular - methods Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Hydro-Lyases - chemistry Hydro-Lyases - genetics Hydro-Lyases - metabolism Hydrolysis Nitriles - chemistry Nitriles - metabolism Protein Engineering - methods Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Rhodococcus - classification Rhodococcus - enzymology Rhodococcus - genetics Rhodococcus erythropolis |
| Title | Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from Rhodococcus erythropolis |
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