Incorporation of Non-Canonical Amino Acids into Proteins by Global Reassignment of Sense Codons

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1798; p. 173
• Main Authors: Fang, Katharine Y, Lieblich, Seth A, Tirrell, David A
• Format: Journal Article
• Language: English
• Published: United States 01.01.2018
• Subjects: Protein engineering; Non-canonical amino acids; Recombinant protein; Proteomics

Non-canonical amino acids are finding increasing use in basic and applied research. Proteins that evolved naturally for biological function did so by exploiting the chemistries of the canonical amino acids; however, when proteins are repurposed for biomedical and pharmacological applications, they a...