Anisotropy-Based Nucleosome Repositioning Assay

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1805; p. 333
• Main Authors: Briggs, Koan, Al-Ani, Gada, Eastlund, Allen, Fischer, Christopher J
• Format: Journal Article
• Language: English
• Published: United States 2018
• Subjects: Adenosine Triphosphate - metabolism; Animals; Anisotropy; Binding Sites; Biological Assay - methods; Chromatin Assembly and Disassembly; DNA - metabolism; Hydrolysis; Kinetics; Nucleosomes - metabolism; Substrate Specificity; Time Factors; Xenopus laevis; Sequential n-step mechanism; Chromatin remodeler; Fluorescence anisotropy; Motor protein; Nucleosome repositioning; ATPase

Most eukaryotic DNA is tightly packaged into nucleosomes that render these sequences largely inaccessible for transcription or repair. Molecular motors called chromatin remodelers use an ATP-dependent mechanism to relieve the inhibition of these processes by sliding or disassembling the nucleosomes....