How to Wire the Diaphragm: Wholemount Staining Methods to Analyze Mammalian Respiratory Innervation

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1668; p. 177
• Main Authors: Saller, Maximilian Michael, Alberton, Paolo, Huber, Andrea B, Huettl, Rosa-Eva
• Format: Journal Article
• Language: English
• Published: United States 2017
• Subjects: Animals; Axon Fasciculation; Axon Guidance; Cell Adhesion Molecules - metabolism; Diaphragm - chemistry; Diaphragm - innervation; Embryo, Mammalian; Fluorescent Dyes - chemistry; Mice; Motor Neurons - metabolism; Muscle Development; Optical Imaging; Phrenic Nerve - growth & development; Staining and Labeling - methods; Thorax - chemistry; Thorax - innervation; Muscle development; Diaphragm; Phrenic nerve; Axon guidance; Fasciculation

Direct or indirect impairment of breathing in humans by diseases or environmental factors can either cause long-term disability and pain, or can ultimately result in death. Automatic respiratory centers in the brainstem control the highly structured process of breathing and signal to a specialized g...