Application of 2D-DIGE and iTRAQ Workflows to Analyze CSF in Gliomas

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2044; p. 81
• Main Authors: Rao, Aishwarya A, Mehta, Kanika, Gahoi, Nikita, Srivastava, Sanjeeva
• Format: Journal Article
• Language: English
• Published: United States 2019
• Subjects: Brain Neoplasms - cerebrospinal fluid; Brain Neoplasms - chemistry; Cerebrospinal Fluid Proteins - analysis; Cerebrospinal Fluid Proteins - chemistry; Cerebrospinal Fluid Proteins - isolation & purification; Chemical Fractionation - instrumentation; Chemical Fractionation - methods; Glioma - cerebrospinal fluid; Glioma - chemistry; Humans; Mass Spectrometry; Proteome - chemistry; Proteome - metabolism; Proteome - standards; Proteomics - methods; Proteomics - standards; Software; Staining and Labeling - methods; Tumor Microenvironment - genetics; Two-Dimensional Difference Gel Electrophoresis - methods; Workflow; Cyanine dyes; Sample fractionation; DIGE; Sample preparation; Mass spectrometric analysis; Internal standard; Reaction quenching; iTRAQ; Isobaric labeling

Proteomics is an indispensable tool for disease biomarker discovery. It is widely used for the analysis of biological fluids such as cerebrospinal fluid (CSF), blood, and saliva, which further aids in our understanding of disease incidence and progression. CSF is often the biospecimen of choice in c...