Real-Time PCR for Gene Expression Quantification in Asthma
The quantitative real-time PCR (qPCR) has become the reference technique for studying gene expression in recent years. The application of qPCR to the study of asthma provides very useful information regarding the gene expression mechanisms. The quantification of RNA from cDNA can be performed by usi...
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Published in | Methods in molecular biology (Clifton, N.J.) Vol. 1434; p. 45 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.01.2016
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Abstract | The quantitative real-time PCR (qPCR) has become the reference technique for studying gene expression in recent years. The application of qPCR to the study of asthma provides very useful information regarding the gene expression mechanisms. The quantification of RNA from cDNA can be performed by using fluorescent dyes or specific sequence probes. Here, we describe the protocol to quantify gene expression levels using SYBR Green as fluorescent dye. The protocol starts with the RNA extraction, followed by reverse transcription to obtain cDNA, quantification and finally data analysis. |
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AbstractList | The quantitative real-time PCR (qPCR) has become the reference technique for studying gene expression in recent years. The application of qPCR to the study of asthma provides very useful information regarding the gene expression mechanisms. The quantification of RNA from cDNA can be performed by using fluorescent dyes or specific sequence probes. Here, we describe the protocol to quantify gene expression levels using SYBR Green as fluorescent dye. The protocol starts with the RNA extraction, followed by reverse transcription to obtain cDNA, quantification and finally data analysis. |
Author | García-Solaesa, Virginia García-Sánchez, Asunción Segundo-Val, Ignacio San |
Author_xml | – sequence: 1 givenname: Ignacio San surname: Segundo-Val fullname: Segundo-Val, Ignacio San email: i.sansegundo.val@gmail.com, i.sansegundo.val@gmail.com organization: Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain. i.sansegundo.val@gmail.com – sequence: 2 givenname: Virginia surname: García-Solaesa fullname: García-Solaesa, Virginia organization: Department of Clinical Genetics, University Hospital of Navarra, Pamplona, Navarra, Spain – sequence: 3 givenname: Asunción surname: García-Sánchez fullname: García-Sánchez, Asunción organization: Department of Biomedical and Diagnostic Sciences, University of Salamanca, Salamanca, Spain |
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Keywords | Expression analysis cDNA mRNA qPCR RT-qPCR (reverse transcription-qPCR) Asthma |
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SubjectTerms | Asthma - genetics Fluorescent Dyes - chemistry Gene Expression Profiling - methods Gene Expression Regulation Genetic Predisposition to Disease - genetics Humans Organic Chemicals - chemistry Real-Time Polymerase Chain Reaction - methods RNA, Messenger - analysis RNA, Messenger - chemistry |
Title | Real-Time PCR for Gene Expression Quantification in Asthma |
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