Exact Theory of Optical Tweezers and Its Application to Absolute Calibration

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1486; p. 25
• Main Authors: Dutra, Rafael S, Viana, Nathan B, Neto, Paulo A Maia, Nussenzveig, H Moysés
• Format: Journal Article
• Language: English
• Published: United States 2017
• Subjects: Calibration; Models, Theoretical; Optical Tweezers; Optics and Photonics; Optical tweezers; Absolute calibration; Optical trap forces

Optical tweezers have become a powerful tool for basic and applied research in cell biology. Here, we describe an experimentally verified theory for the trapping forces generated by optical tweezers based on first principles that allows absolute calibration. For pedagogical reasons, the steps that l...