From Plant Extract to a cDNA Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1405; p. 43
• Main Authors: de Costa, Fernanda, Barber, Carla J S, Reed, Darwin W, Covello, Patrick S
• Format: Journal Article
• Language: English
• Published: United States 01.01.2016
• Subjects: Centella - chemistry; Centella - genetics; Centella - metabolism; Computational Biology; DNA, Complementary; Enzyme Activation; Gene Expression Profiling - methods; Glucosyltransferases - chemistry; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; Plant Extracts; Plant Leaves - chemistry; Plant Leaves - genetics; Plant Leaves - metabolism; Plants, Medicinal - chemistry; Plants, Medicinal - genetics; Plants, Medicinal - metabolism; Proteomics - methods; Saponins - biosynthesis; Centella asiatica; Transcriptomics; Glucosyltransferase; Proteomics; Saponin

Centella asiatica (L.) Urban (Apiaceae), a small annual plant that grows in India, Sri Lanka, Malaysia, and other parts of Asia, is well-known as a medicinal herb with a long history of therapeutic uses. The bioactive compounds present in C. asiatica leaves include ursane-type triterpene sapogenins...