Sialylation levels influence oligosaccharide quantitation: analyzing response variability using high-pH anion-exchange chromatography and pulsed amperometric detection

• Published in: Biopharm International Vol. 16; no. 6; p. 44
• Main Authors: Ganesa, Chandrashekar, Granda, Brian W, Mattaliano, Robert J
• Format: Magazine Article Trade Publication Article
• Language: English
• Published: Monmouth Junction Intellisphere, LLC 01.06.2003; MultiMedia Healthcare Inc
• Subjects: Acids; Analysis; Caustic soda; Cells; Cellulose; Chromatography; Composition; Electrodes; Ions; Molecular weight; Numerical analysis; Oligosaccharides; Proteins; Sensors; Sodium; United States

A protein's carbohydrate side chains affect its solubility, how it folds, its in vivo biological functions, and its pharmacokinetic profiles. Because of the complex nature of glycoproteins and the various recombinant DNA technology processes used to produce them, it remains a challenge to asses...