Fabrication of Molecular Strain Probes for Illuminating Protein-Protein Interactions

A unique bioluminescent imaging probe is introduced for illuminating molecular tension appended by protein-protein interactions (PPIs) of interest. A full-length luciferase is sandwiched between two proteins of interest via minimal flexible linkers. The ligand-activated PPIs append intramolecular te...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1461; p. 175
Main Authors Kim, Sung-Bae, Fujii, Rika
Format Journal Article
LanguageEnglish
Published United States 2016
Subjects
Animals
Cercopithecus aethiops
COS Cells
Ligands
Luciferases - chemistry
Luciferases - genetics
Luciferases - metabolism
Luminescent Agents - chemistry
Luminescent Agents - metabolism
Luminescent Measurements - methods
Molecular Imaging - methods
Molecular Probes - genetics
Molecular Probes - metabolism
Protein Conformation
Protein Interaction Mapping - methods
Structure-Activity Relationship
Molecular tension
Bioluminescence
Protein–protein interactions
Luciferase
Online AccessGet more information

Cover

More Information
Summary:A unique bioluminescent imaging probe is introduced for illuminating molecular tension appended by protein-protein interactions (PPIs) of interest. A full-length luciferase is sandwiched between two proteins of interest via minimal flexible linkers. The ligand-activated PPIs append intramolecular tension to the sandwiched luciferase, boosting or dropping the enzymatic activity in a quantitative manner. This method guides construction of a new lineage of bioassays for ligand-activated PPIs.
ISSN:1940-6029
DOI:10.1007/978-1-4939-3813-1_14