Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging of Lipids in the Ischemic Rat Brain Section: A Practical Approach

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2306; p. 299
• Main Author: Wang, Hay-Yan J
• Format: Journal Article
• Language: English
• Published: United States 2021
• Subjects: Tissue handling and preparation; Matrix-assisted laser desorption/ionization-mass spectrometry imaging; Tissue section drip-wash; Ischemic stroke; Matrix sublimation; Frozen section; Brain tissue lipids

Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) of lipids is considered one of the shotgun lipidomic techniques that explores the in situ distribution of lipids in tissue sections. To successfully perform this task, knowledge and experience in the conventional cryos...