EFFECT OF BROCCOLI SPROUT EXTRACT ON BLOOD LEVELS OF ADENOSINE TRIPHOSPHATE AND REACTIVE OXYGEN SPECIES: AN ACUTE PLACEBO-CONTROLLED PILOT STUDY

During the last decade, broccoli (Brassica oleracea L.) has been recognized as a functional food that potentially confers numerous health benefits. Broccoli contains a glucosinolate, glucoraphanin (4-methylsulfinylbutyl glucosinolate) as well as other glucosinolates, with health-promoting effects. B...

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Published inCurrent topics in nutraceuticals research Vol. 15; no. 1; p. 9
Main Authors Reyes-Izquierdo, Tania, Nemzer, Boris, Argumedo, Ruby, Pietrzkowski, Zbigniew
Format Journal Article
LanguageEnglish
Published New Century Health Publishers, LLC 01.02.2017
Subjects
ATP (Adenosine triphosphate)
Broccoli
Composition
Health aspects
Organic sulfur compounds
Physiological aspects
Plant extracts
Reactive oxygen species
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Summary:During the last decade, broccoli (Brassica oleracea L.) has been recognized as a functional food that potentially confers numerous health benefits. Broccoli contains a glucosinolate, glucoraphanin (4-methylsulfinylbutyl glucosinolate) as well as other glucosinolates, with health-promoting effects. Bioavailability of glucosinolates requires at least three hours to be identified in blood upon ingestion. In this study we tested effect of broccoli-based material on blood level of adenosine triphosphate (ATP) during first two hours upon ingestion. Twenty-two adult healthy subjects were recruited for this study. Subjects were randomly assigned to either Placebo or broccoli sprout extract (BSE) groups (n=11). Blood intracellular ATP levels, glucose, lactate and reactive oxygen species (ROS) were determined throughout the duration of the study. A single dose of 50 mg BSE increased blood levels of ATP up to 55% (p>0.0001) during the duration of the trial. This effect was not associated with any increases in blood ROS or lactate. These results indicate that a single dose of BSE may acutely increase ATP levels in circulating human blood cells without affecting ROS or lactate levels. It is hypothesized that this effect is due to the phytochemicals present in BSE that are other than glucosinolates. Further investigations are justified to identify their phytochemicals. KEY WORDS: ATP, Brassica oleracea L., lactate, Sulforaphane
ISSN:1540-7535