Adenosine Deaminase Activity In Subjects With Altered And Normal Liver Function Profile Test

• Published in: Indian journal of clinical biochemistry Vol. 32; no. S1; p. S232
• Main Authors: Khan, Seraj Ahmad, Lamsal, M, Chaudhari, R.K, Tamang, B, Lamichane, S
• Format: Journal Article
• Language: English
• Published: Springer 24.05.2022
• Subjects: Adenosine; Bilirubin; Comparative analysis; Immune response; Liver; Liver diseases; Medical research; Medicine, Experimental; Phosphatases
• ISSN: 0970-1915

INTRODUCTION: Adenosine Deaminase (ADA) is a nonspecific marker for cellular immunity, is raised in diseases eliciting a cell-mediated immune response. High serum ADA activities were observed in patients with different liver diseases. In this study we aim to determine the association of serum ADA and transaminases levels in liver disease patients and normal control. Methodology: In this comparative cross sectional study 80 subjects, 40 with liver disease and 40 without liver disease were enrolled. Blood samples were drawn and analysis was done. Liver function profile was done by Autoanalyzer (cobas 311, Germany) and ADA activity was measured by Giusti and Gallanti method. Independent t test and Mann Whitney U test for association; Pearson's correlation and Spearman's Rho for correlation study. Multiple linear regression model use for independent association of the variable with ADA. P<0.05 set as statistically significant. RESULTS: Based on liver function profile subjects were divided into Group 1 (normal liver function) and Group 2 (altered liver function) with 40 individual in each group and mean age of 40.0 [+ or -] 16.5 years and 42.4 [+ or -] 16.3 years respectively. Gender were uniformly distributed. Total bilirubin, direct bilirubin, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and adenosine deaminase was higher in group 2 and was statistically significant. ADA was significantly correlated with albumin, AST, ALT and ALP in subjects with normal liver function whereas it was significantly correlated only with direct bilirubin in subjects with altered liver function. Conclusion: Serum ADA activity is significantly raised in subjects with altered liver function profile. KEYWORDS: Adenosine Deaminase, Aspartate Transaminase, Alanine Transaminase, Alkaline Phosphatase.