Standardization and application of modified counter immuno-electrophoresis for the detection of antigenic response against Haemonchus contortus in rabbits

Present paper reports the standardization and application of modified counter immuno-electrophoresis (MCIE) technique to detect the antigenic response of Haemonchus (H.) contortus in rabbits. Sonicated somatic antigen prepared from the freshly collected worms was used to raise hyper immune sera in A...

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Published inPakistan journal of life and social sciences Vol. 9; no. 1; pp. 13 - 16
Main Authors Imtiaz, F. (University of Agriculture, Faisalabad (Pakistan). Dept. of Parasitology), Akhtar, M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Parasitology), Awais, M.M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Parasitology), Muhammad, F. (University of Agriculture, Faisalabad (Pakistan). Dept. of Physiology and Pharmacology), Jamil, H. (University of Agriculture, Faisalabad (Pakistan). Dept. of Theriogenology)
Format Journal Article
LanguageEnglish
Published 30.06.2011
Subjects
ANTIGENS
HAEMONCHUS CONTORTUS
IMMUNE RESPONSE
IMMUNOELECTROPHORESIS
RABBITS
STANDARDIZING
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Summary:Present paper reports the standardization and application of modified counter immuno-electrophoresis (MCIE) technique to detect the antigenic response of Haemonchus (H.) contortus in rabbits. Sonicated somatic antigen prepared from the freshly collected worms was used to raise hyper immune sera in Albino rabbits. Optimum conditions for MCIE were determined by using hyper immune sera in comparison with the negative control. Optimum time for MCIE was 30 minutes at which visible precipitation band(s) were observed with hyper immune sera samples. A flow of 3 volts for 30 minutes was best for obtaining clear results. The quality of the band(s) was dependent upon the concentration of the antigen; and the amount of antigen and antibody on each side of MCIE tube. MCIE is a simple, rapid, and inexpensive test and can be used for preliminary screening of haemonchosis and other parasitic infections in sheep/goats.
Bibliography:CATPAK-75814
L73
ISSN:1727-4915