Detection and Quantification of Major Royal Jelly Protein 1 in Honeybees by ELISA using a Monoclonal Antibody

The most abundant protein in royal jelly (RJ) is the major royal jelly protein 1 (MRJP1), which is a key protein responsible for rapid growth in bee larvae. It plays a pivotal role initiating and maintaining the immune response though the production of inflammatory cytokines. An ELISA method was dev...

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Bibliographic Details
Published inKorean Journal of Apiculture Vol. 26; no. 1
Main Authors Nguyen, Phu Van, Kyonggi University, Suwon, Republic of Korea, No, J.N., Kyonggi University, Suwon, Republic of Korea, Lee, B.R., Kyonggi University, Suwon, Republic of Korea, Yoo, M.S., Kyonggi University, Suwon, Republic of Korea, Yoon, B.S., Kyonggi University, Suwon, Republic of Korea
Format Journal Article
LanguageEnglish
Published 01.04.2011
Subjects
ELISA
GELEE ROYALE
JALEA REAL
MRJP1
ROYAL JELLY
TEST ELISA
Online AccessGet more information
ISSN1225-0252

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Summary:The most abundant protein in royal jelly (RJ) is the major royal jelly protein 1 (MRJP1), which is a key protein responsible for rapid growth in bee larvae. It plays a pivotal role initiating and maintaining the immune response though the production of inflammatory cytokines. An ELISA method was developed to detect and quantify MRJP1 using a specific monoclonal anti-MRJP1 antibody. The limit of detection for MRJP1 was 1ng/ml, MRJP1 was identified in RJ samples collected from Vietnam and Korea, and under storage conditions. The results showed that the ELISA had good sensitivity and precision for detecting MRJP1 in RJ samples.
Bibliography:2012004565
L01
ISSN:1225-0252