motif in the C-terminal domain of {phi}C31 integrase controls the directionality of recombination

• Published in: Nucleic acids research Vol. 36; no. 12; pp. 3879 - 3891
• Main Authors: Rowley, Paul A, Smith, Matthew C.A, Younger, Ellen, Smith, Margaret C.M
• Format: Journal Article
• Language: English
• Published: Oxford Oxford Publishing Limited (England) 01.07.2008

Bacteriophage {phi}C31 encodes an integrase, which acts on the phage and host attachment sites, attP and attB, to form an integrated prophage flanked by attL and attR. In the absence of accessory factors, {phi}C31 integrase cannot catalyse attL x attR recombination to excise the prophage. To underst...