IDENTIFICACIÓN DEL GEN syl EN AISLAMIENTOS CUBANOS Streptococcus suis PROCEDENTES DE CERDOS MEDIANTE LA REACCIÓN EN CADENA DE LA POLIMERASA (PCR)
Streptococcus suis es un coco anaerobio facultativo, considerado un importante agente asociado a meningitis, artritis, endocarditis y neumonía en cerdos. En este trabajo se realizó el aislamiento de S.suis a partir de diferentes condiciones clínicas como neumonía, abscesos en articulaciones y tonsil...
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| Published in | Revista de salud animal Vol. 32; no. 1; pp. 48 - 53 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | Portuguese Spanish |
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Centro Nacional de Sanidad Agropecuaria
01.04.2010
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| ISSN | 0253-570X 2224-4700 2224-4700 |
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| Abstract | Streptococcus suis es un coco anaerobio facultativo, considerado un importante agente asociado a meningitis, artritis, endocarditis y neumonía en cerdos. En este trabajo se realizó el aislamiento de S.suis a partir de diferentes condiciones clínicas como neumonía, abscesos en articulaciones y tonsilas de animales asintomáticos. Se identificaron 14 cepas de S. suis: 11 a partir de pulmones, 2 de abscesos y 1 de tonsila. Las cepas mostraron diferentes patrones de pruebas bioquímicas. El gen que codifica para la hemolisina denominados suilisina (syl) se detectó por PCR solamente en 5 cepas de las cuales 4 proceden de pulmón y una de tonsila. Sin embargo, en los aislamientos procedentes de abscesos y asociados a artritis no se detectó la presencia de este gen, lo cual muestra un comportamiento similar a las cepas presentes en Norte América syl -, asociadas a enfermedad invasiva.
Streptococcus suis is a facultative anaerobic coccus considered an important agent associated to meningitis, arthritis, endocarditis and pneumonia. In this work, the isolation of S. suis from different clinical conditions such as pneumonia, joint and tonsil in asymptomatic animals was carried out. Fourteen strains of S. suis were identified: 11 from lungs, 2 from abscess and 1 from tonsils. The strains showed different pattern of biochemistry test. The syl gene codifying for suylisin was detected by PCR only in 5 strains, four coming from lung and one from tonsil. However, in the isolations from abscess and associated to arthritis, the presence of this gene was not detected showing a similar behaviour to the strains from North America (syl -) associated to invasive disease. |
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| AbstractList | Streptococcus suis es un coco anaerobio facultativo, considerado un importante agente asociado a meningitis, artritis, endocarditis y neumonía en cerdos. En este trabajo se realizó el aislamiento de S.suis a partir de diferentes condiciones clínicas como neumonía, abscesos en articulaciones y tonsilas de animales asintomáticos. Se identificaron 14 cepas de S. suis: 11 a partir de pulmones, 2 de abscesos y 1 de tonsila. Las cepas mostraron diferentes patrones de pruebas bioquímicas. El gen que codifica para la hemolisina denominados suilisina (syl) se detectó por PCR solamente en 5 cepas de las cuales 4 proceden de pulmón y una de tonsila. Sin embargo, en los aislamientos procedentes de abscesos y asociados a artritis no se detectó la presencia de este gen, lo cual muestra un comportamiento similar a las cepas presentes en Norte América syl -, asociadas a enfermedad invasiva. Streptococcus suis es un coco anaerobio facultativo, considerado un importante agente asociado a meningitis, artritis, endocarditis y neumonía en cerdos. En este trabajo se realizó el aislamiento de S.suis a partir de diferentes condiciones clínicas como neumonía, abscesos en articulaciones y tonsilas de animales asintomáticos. Se identificaron 14 cepas de S. suis: 11 a partir de pulmones, 2 de abscesos y 1 de tonsila. Las cepas mostraron diferentes patrones de pruebas bioquímicas. El gen que codifica para la hemolisina denominados suilisina (syl) se detectó por PCR solamente en 5 cepas de las cuales 4 proceden de pulmón y una de tonsila. Sin embargo, en los aislamientos procedentes de abscesos y asociados a artritis no se detectó la presencia de este gen, lo cual muestra un comportamiento similar a las cepas presentes en Norte América syl -, asociadas a enfermedad invasiva. Streptococcus suis is a facultative anaerobic coccus considered an important agent associated to meningitis, arthritis, endocarditis and pneumonia. In this work, the isolation of S. suis from different clinical conditions such as pneumonia, joint and tonsil in asymptomatic animals was carried out. Fourteen strains of S. suis were identified: 11 from lungs, 2 from abscess and 1 from tonsils. The strains showed different pattern of biochemistry test. The syl gene codifying for suylisin was detected by PCR only in 5 strains, four coming from lung and one from tonsil. However, in the isolations from abscess and associated to arthritis, the presence of this gene was not detected showing a similar behaviour to the strains from North America (syl -) associated to invasive disease. |
| Author | Espinosa, Ivette(Centro Nacional de Sanidad Agropecuaria (CENSA) Dpto. Biología Molecular) Lobo, Evelyn(Centro Nacional de Sanidad Agropecuaria (CENSA) Dpto. Biología Molecular) Domínguez, Patricia(Centro Nacional de Sanidad Agropecuaria (CENSA) Dpto. Biología Molecular) Alfonso, P(Centro Nacional de Sanidad Agropecuaria (CENSA) Dpto. Biología Molecular) Martínez, Siomara(Centro Nacional de Sanidad Agropecuaria (CENSA) Dpto. Biología Molecular) |
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| Keywords | suilysine pigs suilisina Streptococcu suis Streptococcus suis cerdos PCR |
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| Title | IDENTIFICACIÓN DEL GEN syl EN AISLAMIENTOS CUBANOS Streptococcus suis PROCEDENTES DE CERDOS MEDIANTE LA REACCIÓN EN CADENA DE LA POLIMERASA (PCR) |
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