Clinical biochemistry parameters in C57BL/6J mice after blood collection from the submandibular vein and retroorbital plexus

Collection of blood from the submandibular vein allows simple and rapid processing of many animals without anesthesia and facilitates rapid recovery with no signs of pain and discomfort in the mice. Here we compared the submandibular vein and retroorbital plexus blood collection methods, to determin...

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Published inJournal of the American Association for Laboratory Animal Science Vol. 49; no. 2; pp. 202 - 206
Main Authors Fernandez, Itziar, Pena, Arantza, Teso, Nahia Del, Perez, Virginia, Rodriguez-Cuesta, Juan
Format Journal Article
LanguageEnglish
Published United States American Association for Laboratory Animal Science 01.03.2010
Subjects
Alanine Transaminase - blood
Animals
Aspartate Aminotransferases - blood
blood chemistry
Blood Glucose - analysis
Blood Proteins - analysis
blood sampling
Blood Specimen Collection - adverse effects
Blood Specimen Collection - methods
Blood Specimen Collection - veterinary
blood veins
Cholesterol - blood
Creatinine - blood
Experimental Use
laboratory animals
Male
Mice
Mice, Inbred C57BL - blood
Orbit - blood supply
Reference Values
Serum Albumin - analysis
Submandibular Gland - blood supply
Triglycerides - blood
Veins
venipuncture
Online AccessGet full text
ISSN1559-6109
2769-6677
2769-6677

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Abstract Collection of blood from the submandibular vein allows simple and rapid processing of many animals without anesthesia and facilitates rapid recovery with no signs of pain and discomfort in the mice. Here we compared the submandibular vein and retroorbital plexus blood collection methods, to determine the potential effect of the sampling technique on several clinical biochemistry parameters in C57BL/6J mice. We found statistically significant differences for 8 of the 9 biochemical parameters studied between the 2 blood sampling techniques. Compared with samples collected from the retroorbital plexus, blood obtained from the submadibular vein had higher levels of AST, ALT, protein, albumin, triglycerides, total cholesterol, and creatinine. Glucose values of retroorbital blood were higher than those from the submandibular vein. Urea levels were similar for both sampling techniques. Our results demonstrate that the technique used to obtain blood samples affects parameters commonly used to assess animal health. We recommend caution when comparing results of biochemical analysis of blood obtained from the submandibular vein in mice with reference values obtained by other blood sampling techniques.
AbstractList Collection of blood from the submandibular vein allows simple and rapid processing of many animals without anesthesia and facilitates rapid recovery with no signs of pain and discomfort in the mice. Here we compared the submandibular vein and retroorbital plexus blood collection methods, to determine the potential effect of the sampling technique on several clinical biochemistry parameters in C57BL/6J mice. We found statistically significant differences for 8 of the 9 biochemical parameters studied between the 2 blood sampling techniques. Compared with samples collected from the retroorbital plexus, blood obtained from the submadibular vein had higher levels of AST, ALT, protein, albumin, triglycerides, total cholesterol, and creatinine. Glucose values of retroorbital blood were higher than those from the submandibular vein. Urea levels were similar for both sampling techniques. Our results demonstrate that the technique used to obtain blood samples affects parameters commonly used to assess animal health. We recommend caution when comparing results of biochemical analysis of blood obtained from the submandibular vein in mice with reference values obtained by other blood sampling techniques.
Collection of blood from the submandibular vein allows simple and rapid processing of many animals without anesthesia and facilitates rapid recovery with no signs of pain and discomfort in the mice. Here we compared the submandibular vein and retroorbital plexus blood collection methods, to determine the potential effect of the sampling technique on several clinical biochemistry parameters in C57BL/6J mice. We found statistically significant differences for 8 of the 9 biochemical parameters studied between the 2 blood sampling techniques. Compared with samples collected from the retroorbital plexus, blood obtained from the submadibular vein had higher levels of AST, ALT, protein, albumin, triglycerides, total cholesterol, and creatinine. Glucose values of retroorbital blood were higher than those from the submandibular vein. Urea levels were similar for both sampling techniques. Our results demonstrate that the technique used to obtain blood samples affects parameters commonly used to assess animal health. We recommend caution when comparing results of biochemical analysis of blood obtained from the submandibular vein in mice with reference values obtained by other blood sampling techniques.
Collection of blood from the submandibular vein allows simple and rapid processing of many animals without anesthesia and facilitates rapid recovery with no signs of pain and discomfort in the mice. Here we compared the submandibular vein and retroorbital plexus blood collection methods, to determine the potential effect of the sampling technique on several clinical biochemistry parameters in C57BL/6J mice. We found statistically significant differences for 8 of the 9 biochemical parameters studied between the 2 blood sampling techniques. Compared with samples collected from the retroorbital plexus, blood obtained from the submandibular vein had higher levels of AST, ALT, protein, albumin, triglycerides, total cholesterol, and creatinine. Glucose values of retroorbital blood were higher than those from the submandibular vein. Urea levels were similar for both sampling techniques. Our results demonstrate that the technique used to obtain blood samples affects parameters commonly used to assess animal health. We recommend caution when comparing results of biochemical analysis of blood obtained from the submandibular vein in mice with reference values obtained by other blood sampling techniques.
Collection of blood from the submandibular vein allows simple and rapid processing of many animals without anesthesia and facilitates rapid recovery with no signs of pain and discomfort in the mice. Here we compared the submandibular vein and retroorbital plexus blood collection methods, to determine the potential effect of the sampling technique on several clinical biochemistry parameters in C57BL/6J mice. We found statistically significant differences for 8 of the 9 biochemical parameters studied between the 2 blood sampling techniques. Compared with samples collected from the retroorbital plexus, blood obtained from the submandibular vein had higher levels of AST, ALT, protein, albumin, triglycerides, total cholesterol, and creatinine. Glucose values of retroorbital blood were higher than those from the submandibular vein. Urea levels were similar for both sampling techniques. Our results demonstrate that the technique used to obtain blood samples affects parameters commonly used to assess animal health. We recommend caution when comparing results of biochemical analysis of blood obtained from the submandibular vein in mice with reference values obtained by other blood sampling techniques.Collection of blood from the submandibular vein allows simple and rapid processing of many animals without anesthesia and facilitates rapid recovery with no signs of pain and discomfort in the mice. Here we compared the submandibular vein and retroorbital plexus blood collection methods, to determine the potential effect of the sampling technique on several clinical biochemistry parameters in C57BL/6J mice. We found statistically significant differences for 8 of the 9 biochemical parameters studied between the 2 blood sampling techniques. Compared with samples collected from the retroorbital plexus, blood obtained from the submandibular vein had higher levels of AST, ALT, protein, albumin, triglycerides, total cholesterol, and creatinine. Glucose values of retroorbital blood were higher than those from the submandibular vein. Urea levels were similar for both sampling techniques. Our results demonstrate that the technique used to obtain blood samples affects parameters commonly used to assess animal health. We recommend caution when comparing results of biochemical analysis of blood obtained from the submandibular vein in mice with reference values obtained by other blood sampling techniques.
Author Fernandez, Itziar
Pena, Arantza
Teso, Nahia Del
Rodriguez-Cuesta, Juan
Perez, Virginia
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– reference: 18459706 - J Am Assoc Lab Anim Sci. 2008 May;47(3):8-15
– reference: 15669134 - Contemp Top Lab Anim Sci. 2004 Nov;43(6):42-51
– reference: 15134363 - Comp Med. 2004 Apr;54(2):176-8
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– reference: 9807749 - Lab Anim. 1998 Oct;32(4):364-8
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– reference: 15520880 - Mamm Genome. 2004 Oct;15(10):768-83
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SubjectTerms Alanine Transaminase - blood
Animals
Aspartate Aminotransferases - blood
blood chemistry
Blood Glucose - analysis
Blood Proteins - analysis
blood sampling
Blood Specimen Collection - adverse effects
Blood Specimen Collection - methods
Blood Specimen Collection - veterinary
blood veins
Cholesterol - blood
Creatinine - blood
Experimental Use
laboratory animals
Male
Mice
Mice, Inbred C57BL - blood
Orbit - blood supply
Reference Values
Serum Albumin - analysis
Submandibular Gland - blood supply
Triglycerides - blood
Veins
venipuncture
Title Clinical biochemistry parameters in C57BL/6J mice after blood collection from the submandibular vein and retroorbital plexus
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