beta-Glucosidase activity from the thermophilic fungus Scytalidium thermophilum is stimulated by glucose and xylose

• Published in: FEMS microbiology letters Vol. 240; no. 2; pp. 137 - 143
• Main Authors: Zanoelo, F.F, Polizeli, M. de L.T. de M, Terenzi, H.F, Jorge, J.A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 15.11.2004; Blackwell; Oxford University Press
• Subjects: Action of physical and chemical agents; Ascomycota - enzymology; beta-Glucosidase - chemistry; beta-Glucosidase - isolation & purification; beta-Glucosidase - metabolism; Biological and medical sciences; Carbohydrates; Cellobiase; Cellobiose; Cellobiose - metabolism; Disaccharides - metabolism; Enzyme Activators - pharmacology; Enzyme Induction; Enzyme Stability; Enzymes; Fundamental and applied biological sciences. Psychology; fungus physiology; Glucose; Glucose - pharmacology; Glucose‐exhanced β‐glucosidase; Glucosidase; Glucosides - metabolism; Glycosides - metabolism; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Hydrogen-Ion Concentration; Isoelectric Point; Lactose; Lactose - metabolism; Microbiology; Molecular Weight; Mycelia; Mycology; Nitrophenylgalactosides - metabolism; p-Nitrophenyl-b-D-glucopyranoside; Scytalidium thermophilum; Substrate Specificity; Substrates; Temperature; Thermophilic fungi; Xylopyranoside; Xylose; Xylose - pharmacology; Xylose‐exhanced β‐glucosidase; β-Glucosidase; β‐Glycosidase; β-Glucosidase; Glucose-exhanced β-glucosidase; Cellobiase; β-Glycosidase; Xylose-exhanced β-glucosidase; Thermoproteales; Filtration; Archaeobacteria; Enzyme; Thermophily; Glucose; Fungi; Xylose; Thermophilum; Glycosidases; Bacteria; Hydrolases; Thermoproteaceae; Electrophoresis; Fungi Imperfecti; Scytalidium; O-Glycosidases; Thallophyta

An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively. The enzyme was stable up to 1...