Dynamic Ca²⁺-Dependent Stimulation of Vesicle Fusion by Membrane-Anchored Synaptotagmin 1
In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca²⁺ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca²⁺-triggered membrane fusion. Using an in vitro singl...
Saved in:
| Published in | Science (American Association for the Advancement of Science) Vol. 328; no. 5979; pp. 760 - 763 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Washington, DC
American Association for the Advancement of Science
07.05.2010
The American Association for the Advancement of Science |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca²⁺ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca²⁺-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca²⁺ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca²⁺ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca²⁺ levels and may function as a dynamic presynaptic Ca²⁺ sensor to control the probability of neurotransmitter release. |
|---|---|
| AbstractList | In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca2+-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca2+ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca2+ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca2+ levels and may function as a dynamic presynaptic Ca2+ sensor to control the probability of neurotransmitter release. In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca²₊ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca²₊ -triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Sytl enhanced Ca²₊ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca²₊ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca²₊ levels and may function as a dynamic presynaptic Ca²₊ sensor to control the probability of neurotransmitter release. In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca super(2+) levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca super(2+)-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca super(2+) sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca super(2+) level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca super(2+) levels and may function as a dynamic presynaptic Ca super(2+) sensor to control the probability of neurotransmitter release. In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca2+-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca2+ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca2+ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca2+ levels and may function as a dynamic presynaptic Ca2+ sensor to control the probability of neurotransmitter release. [PUBLICATION ABSTRACT] In neurons, synaptotagmin1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca 2+ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca 2+ -triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca 2+ -sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca 2+ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca 2+ levels, and may function as a dynamic presynaptic Ca 2+ sensor to control the probability of neurotransmitter release. In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca²⁺ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca²⁺-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca²⁺ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca²⁺ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca²⁺ levels and may function as a dynamic presynaptic Ca²⁺ sensor to control the probability of neurotransmitter release. |
| Author | Yang, Yoosoo Kweon, Dae-Hyuk Lee, Han-Ki Su, Zengliu Lee, Tae-Sun Hyeon, Changbong Lee, Hong-Won Shin, Yeon-Kyun Yoon, Tae-Young |
| AuthorAffiliation | 5 School of Computational Sciences, Korea Institute for Advanced Study, Seoul 130-722, South Korea 4 Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 4152 Molecular Biology Building, Ames, Iowa 50011, USA 3 Department of Genetic Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do 4400-746, South Korea 6 Division of Integrative Biosciences and Biotechnology, POSTECH, Pohang 790-784, South Korea 2 Institute for the BioCentury, KAIST, Daejeon 305-701, South Korea 1 Department of Physics, KAIST, Daejeon 305-701, South Korea |
| AuthorAffiliation_xml | – name: 3 Department of Genetic Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do 4400-746, South Korea – name: 5 School of Computational Sciences, Korea Institute for Advanced Study, Seoul 130-722, South Korea – name: 2 Institute for the BioCentury, KAIST, Daejeon 305-701, South Korea – name: 4 Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, 4152 Molecular Biology Building, Ames, Iowa 50011, USA – name: 1 Department of Physics, KAIST, Daejeon 305-701, South Korea – name: 6 Division of Integrative Biosciences and Biotechnology, POSTECH, Pohang 790-784, South Korea |
| Author_xml | – sequence: 1 fullname: Lee, Han-Ki – sequence: 2 fullname: Yang, Yoosoo – sequence: 3 fullname: Su, Zengliu – sequence: 4 fullname: Hyeon, Changbong – sequence: 5 fullname: Lee, Tae-Sun – sequence: 6 fullname: Lee, Hong-Won – sequence: 7 fullname: Kweon, Dae-Hyuk – sequence: 8 fullname: Shin, Yeon-Kyun – sequence: 9 fullname: Yoon, Tae-Young |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22744314$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/20448186$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFkcuKFDEYhYOMOD2ja1dqIYir0twvG2HocVQYcdGOKyGkUqmeNFVJm1QJvfSVXLr0UXwSU3Q7XjYuwk9yPg4n5z8BRyEGB8B9BJ8hhPnzbL0L1pWLFALjW2CBoGK1wpAcgQWEhNcSCnYMTnLeQFg0Re6AYwwplUjyBfh4vgtm8LZamu9ff3z5Vp-7rQutC2O1Gv0w9Wb0MVSxqz647G3vqospzy_NrnrrhiaZ4OqzYK9jcm21KmbbMY5mPfhQobvgdmf67O4d5im4unj5fvm6vnz36s3y7LLuKOZjTQ1BjljRcKFaTiwznWoaTOfkpoGUo1YoRxghGEKJOGUdZp2lkDNuoKDkFLzY-26nZnCtLemT6fU2-cGknY7G67-V4K_1On7WWCnKqCoGTw8GKX6aXB714LN1fV9-F6esBeVQkvn8lyQlJxYMF_LxP-QmTimUHjRGxYtIxQr08M_kN5F_LagATw6Aydb0Xenb-vybw4JSguYKHuy5TR5jutHnjpiUouiP9npnojbrVDyuVhgiApEkkhJMfgLywLVr |
| CODEN | SCIEAS |
| ContentType | Journal Article |
| Copyright | 2010 American Association for the Advancement of Science 2015 INIST-CNRS Copyright © 2010, American Association for the Advancement of Science |
| Copyright_xml | – notice: 2010 American Association for the Advancement of Science – notice: 2015 INIST-CNRS – notice: Copyright © 2010, American Association for the Advancement of Science |
| DBID | FBQ IQODW CGR CUY CVF ECM EIF NPM 7QF 7QG 7QL 7QP 7QQ 7QR 7SC 7SE 7SN 7SP 7SR 7SS 7T7 7TA 7TB 7TK 7TM 7U5 7U9 8BQ 8FD C1K F28 FR3 H8D H8G H94 JG9 JQ2 K9. KR7 L7M L~C L~D M7N P64 RC3 7X8 5PM |
| DOI | 10.1126/science.1187722 |
| DatabaseName | AGRIS Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Aluminium Industry Abstracts Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Ceramic Abstracts Chemoreception Abstracts Computer and Information Systems Abstracts Corrosion Abstracts Ecology Abstracts Electronics & Communications Abstracts Engineered Materials Abstracts Entomology Abstracts (Full archive) Industrial and Applied Microbiology Abstracts (Microbiology A) Materials Business File Mechanical & Transportation Engineering Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Solid State and Superconductivity Abstracts Virology and AIDS Abstracts METADEX Technology Research Database Environmental Sciences and Pollution Management ANTE: Abstracts in New Technology & Engineering Engineering Research Database Aerospace Database Copper Technical Reference Library AIDS and Cancer Research Abstracts Materials Research Database ProQuest Computer Science Collection ProQuest Health & Medical Complete (Alumni) Civil Engineering Abstracts Advanced Technologies Database with Aerospace Computer and Information Systems Abstracts Academic Computer and Information Systems Abstracts Professional Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Materials Research Database Technology Research Database Computer and Information Systems Abstracts – Academic Mechanical & Transportation Engineering Abstracts Nucleic Acids Abstracts ProQuest Computer Science Collection Computer and Information Systems Abstracts ProQuest Health & Medical Complete (Alumni) Materials Business File Environmental Sciences and Pollution Management Aerospace Database Copper Technical Reference Library Engineered Materials Abstracts Genetics Abstracts Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Advanced Technologies Database with Aerospace ANTE: Abstracts in New Technology & Engineering Civil Engineering Abstracts Aluminium Industry Abstracts Virology and AIDS Abstracts Electronics & Communications Abstracts Ceramic Abstracts Ecology Abstracts Neurosciences Abstracts METADEX Biotechnology and BioEngineering Abstracts Computer and Information Systems Abstracts Professional Entomology Abstracts Animal Behavior Abstracts Solid State and Superconductivity Abstracts Engineering Research Database Calcium & Calcified Tissue Abstracts Corrosion Abstracts MEDLINE - Academic |
| DatabaseTitleList | MEDLINE Calcium & Calcified Tissue Abstracts Materials Research Database |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) Biology |
| EISSN | 1095-9203 |
| EndPage | 763 |
| ExternalDocumentID | 2024462541 20448186 22744314 40655887 US201301838432 |
| Genre | Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural Feature |
| GrantInformation_xml | – fundername: NIGMS NIH HHS grantid: R01 GM051290 – fundername: NIGMS NIH HHS grantid: R01 GM051290-16 |
| GroupedDBID | --- --Z -DZ -ET -~X .-4 ..I .55 .DC .GJ .GO .HR 0-V 08G 0B8 0R~ 0WA 123 186 18M 2FS 2KS 2WC 2XV 34G 36B 39C 3EH 3R3 3V. 4.4 41~ 42X 4R4 53G 5RE 63O 66. 68V 692 6OB 6TJ 79B 7X2 7X7 7XC 7~K 85S 88A 88E 88I 8AF 8CJ 8F7 8FE 8FG 8FH 8FI 8FJ 8G5 8GL 8WZ 97F A6W AABCJ AACGO AADHG AAFWJ AAIKC AAJYS AAKAS AAMNW AANCE AAWTO AAYJJ AAYOK ABBHK ABCQX ABDBF ABDEX ABEFU ABIVO ABJCF ABOCM ABPLY ABPMR ABPPZ ABPTK ABQIJ ABTAH ABTLG ABUWG ABWJO ABZEH ACBEA ACBEC ACGFO ACGFS ACGOD ACIWK ACMJI ACNCT ACPRK ACQAM ACQOY ACTDY ADBBV ADDRP ADMHC ADULT ADZCM ADZLD AEGBM AENEX AETEA AEUPB AEXZC AFCHL AFDAS AFFDN AFFNX AFHKK AFKRA AFOSN AFQFN AFRAH AGCDD AGFXO AGNAY AGSOS AHMBA AIDAL AIDUJ AJGZS AJUXI ALMA_UNASSIGNED_HOLDINGS ALSLI ANJGP ARALO ARAPS ASPBG ATCPS AVWKF AZQEC B-7 BBNVY BBWZM BCU BEC BENPR BGLVJ BHPHI BKF BKNYI BKSAR BLC BPHCQ BVXVI C2- C45 C51 CCPQU CJNVE CS3 D0S D1I D1J D1K DB2 DCCCD DNJUQ DOOOF DU5 DWIUU DWQXO D~A EAU EBS EGS EJD EMOBN ESX EWM EX3 F20 F5P FA8 FBQ FEDTE FYUFA G8K GICCO GNUQQ GUQSH GX1 HCIFZ HGD HMCUK HQ3 HTVGU HVGLF HZ~ I.T IAG IAO IBG IEA IEP IER IGG IGS IH2 IHR INH INR IOF IOV IPC IPO IPY ISE ISN ISR ITC J5H J9C JAAYA JBMMH JCF JENOY JHFFW JKQEH JLS JLXEF JPM JSG JSODD JST K-O K6- K9- KB. KCC KQ8 L6V L7B LK5 LK8 LPU LSO LU7 M0K M0L M0P M0R M1P M2O M2P M2Q M7P M7R M7S MQT MVM N4W N9A NEJ NHB O9- OCB OFXIZ OGEVE OK1 OMK OVD P-O P2P P62 PATMY PCBAR PDBOC PK8 PQQKQ PROAC PSQYO PTHSS PV9 PYCSY PZZ QJJ QS- R05 RHF RHI RNS RXW RZL SA0 SC5 SJFOW SJN SKT TAE TEORI TN5 TWZ UBW UBY UCV UHB UHU UKHRP UKR UMD UNMZH UQL USG VOH VQA VVN WH7 WI4 WOQ WOW X7L X7M XFK XIH XJF XKJ XOL XZL Y6R YCJ YJ6 YK4 YKV YNT YOJ YR2 YRY YSQ YV5 YWH YXB YYP YYQ YZZ ZA5 ZCA ZCF ZCG ZE2 ZGI ZKG ZVL ZVM ZXP ZY4 ~02 ~G0 ~H1 ~KM ~ZZ ABXSQ AHPSJ AQVQM 08R AAUGY IQODW ADACV ADUKH AFRQD ALIPV CGR CUY CVF ECM EIF IPSME NPM UIG 7QF 7QG 7QL 7QP 7QQ 7QR 7SC 7SE 7SN 7SP 7SR 7SS 7T7 7TA 7TB 7TK 7TM 7U5 7U9 8BQ 8FD C1K F28 FR3 H8D H8G H94 JG9 JQ2 K9. KR7 L7M L~C L~D M7N P64 RC3 7X8 5PM |
| ID | FETCH-LOGICAL-f426t-4a31e3c7b679d63c5af9bb248075ab0461d79e353320081645f25fc40656a0743 |
| ISSN | 0036-8075 |
| IngestDate | Tue Sep 17 21:26:26 EDT 2024 Fri Oct 25 21:33:59 EDT 2024 Fri Oct 25 01:54:45 EDT 2024 Wed Nov 06 08:55:54 EST 2024 Sat Sep 28 08:16:14 EDT 2024 Sun Oct 29 17:08:31 EDT 2023 Fri Feb 02 07:02:43 EST 2024 Wed Dec 27 19:16:27 EST 2023 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5979 |
| Keywords | Sensitivity Neuron Calcium Synaptic membrane Plasma membrane Synaptic vesicle Stimulation Synaptotagmin Experimental study In vitro Thought Presynaptic |
| Language | English |
| License | CC BY 4.0 |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-f426t-4a31e3c7b679d63c5af9bb248075ab0461d79e353320081645f25fc40656a0743 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. |
| PMID | 20448186 |
| PQID | 213603895 |
| PQPubID | 1256 |
| PageCount | 4 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_2994549 proquest_miscellaneous_746083608 proquest_miscellaneous_733532752 proquest_journals_213603895 pubmed_primary_20448186 pascalfrancis_primary_22744314 jstor_primary_40655887 fao_agris_US201301838432 |
| PublicationCentury | 2000 |
| PublicationDate | 2010-05-07 |
| PublicationDateYYYYMMDD | 2010-05-07 |
| PublicationDate_xml | – month: 05 year: 2010 text: 2010-05-07 day: 07 |
| PublicationDecade | 2010 |
| PublicationPlace | Washington, DC |
| PublicationPlace_xml | – name: Washington, DC – name: United States – name: Washington |
| PublicationTitle | Science (American Association for the Advancement of Science) |
| PublicationTitleAlternate | Science |
| PublicationYear | 2010 |
| Publisher | American Association for the Advancement of Science The American Association for the Advancement of Science |
| Publisher_xml | – name: American Association for the Advancement of Science – name: The American Association for the Advancement of Science |
| References | 12119360 - J Cell Biol. 2002 Jul 22;158(2):273-82 15217342 - Annu Rev Neurosci. 2004;27:509-47 18552825 - Nat Struct Mol Biol. 2008 Jul;15(7):707-13 16491093 - Nat Struct Mol Biol. 2006 Mar;13(3):209-17 18618940 - Nat Struct Mol Biol. 2008 Jul;15(7):665-74 7969419 - Nature. 1994 Nov 3;372(6501):55-63 18644890 - J Cell Biol. 2008 Jul 28;182(2):355-66 18953334 - Nat Struct Mol Biol. 2008 Nov;15(11):1160-8 15044754 - Science. 2004 Apr 16;304(5669):435-8 16782782 - Biophys J. 2006 Sep 1;91(5):1767-77 9529252 - Cell. 1998 Mar 20;92(6):759-72 17167056 - Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19731-6 17478680 - Science. 2007 May 25;316(5828):1205-8 19703397 - Cell. 2009 Aug 21;138(4):709-21 14718921 - Nat Struct Mol Biol. 2004 Jan;11(1):36-44 15919191 - Curr Opin Neurobiol. 2005 Jun;15(3):266-74 17110340 - Cell. 2006 Nov 17;127(4):831-46 19716167 - Cell. 2009 Sep 4;138(5):935-46 18275379 - Annu Rev Biochem. 2008;77:615-41 12062043 - Neuron. 2002 May 16;34(4):599-611 16565726 - Nat Struct Mol Biol. 2006 Apr;13(4):323-30 17891149 - Nat Struct Mol Biol. 2007 Oct;14(10):904-11 19164559 - Proc Natl Acad Sci U S A. 2009 Jan 27;106(4):979-84 18485705 - Curr Biol. 2008 May 20;18(10):715-22 16912714 - Nat Rev Mol Cell Biol. 2006 Sep;7(9):631-43 14744428 - Cell. 2004 Jan 23;116(2):153-66 18046404 - Nature. 2007 Nov 29;450(7170):676-82 17672888 - BMC Genomics. 2007;8:259 19489736 - Annu Rev Biochem. 2009;78:903-28 |
| References_xml | |
| SSID | ssj0009593 |
| Score | 2.380244 |
| Snippet | In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca²⁺ levels rise. However,... In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca²₊ levels rise. However,... In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca2+ levels rise. However,... In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca super(2+) levels rise.... In neurons, synaptotagmin1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca 2+ levels rise. However,... |
| SourceID | pubmedcentral proquest pubmed pascalfrancis jstor fao |
| SourceType | Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 760 |
| SubjectTerms | Animals Biochemistry Biological and medical sciences Calcium - metabolism Cell membranes Cellular biology Fluorescence Fundamental and applied biological sciences. Psychology Imaging Kinetics Lipids Magnesium - metabolism Membrane Fusion Membrane Lipids - metabolism Membranes Neurons Neurotransmitter Agents - metabolism Neurotransmitters Phosphatidylinositol 4,5-Diphosphate - metabolism Physiological stimulation Proteins Rats rev genes SNARE Proteins - metabolism Synaptic Vesicles - physiology Synaptotagmin I - chemistry Synaptotagmin I - metabolism Synaptotagmins Vertebrates: nervous system and sense organs |
| Title | Dynamic Ca²⁺-Dependent Stimulation of Vesicle Fusion by Membrane-Anchored Synaptotagmin 1 |
| URI | https://www.jstor.org/stable/40655887 https://www.ncbi.nlm.nih.gov/pubmed/20448186 https://www.proquest.com/docview/213603895 https://search.proquest.com/docview/733532752 https://search.proquest.com/docview/746083608 https://pubmed.ncbi.nlm.nih.gov/PMC2994549 |
| Volume | 328 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9NAEF6FIiQuiBZKTaHaA0igyJW9u7bjY-iDCLXl0ASlJ2vXXrc5xK6wcwg_gt_MTHbtOLQ8L1YU2-vE8-08dma_IeQN_Lg0k5q7oc4yV4BD6w5imbkqzpSXKRFEKS7on1-Eo4n4NA2mvd73TtXSolaH6bd795X8j1ThO5Ar7pL9B8m2g8IX8BnkC0eQMBz_SsbHpp18_0iyt-yDe2wb2tbgQs7mti8XeoNfdIW39k8XuDaGHue5nkOYXGh3WID-wxr0SxjrtsYk1XxW9P2u09rMf3BG2wRPR6xtpeLQ1BM05QX2ts5ag636GUnsWtzqG7tifVWWVVmuk1SrtInGXcaLFntL3RYJFNeqtFbXLlqYfLvpbtsoYsuDbMyQ0b0eto1kHu8qZ84GHRRC9BN3tG1kWhFYw2015V2b0OliqQ-xvXpktkJvsm9ffE5OJ2dnyfhkOn5AHjJQXKgxP079X5I4W6qoziasZnjwVHJZNjWuWHArK5hzuWmWcl8083NRbsfLGT8lT2x4QocGa9ukp4sd8sg0LF3ukG0r04q-s3zl75-RKwtDCjDsr0FIOyCkZU4tCKkBIVVLegeEdAOE1H9OJqcn46ORazt2uDl4erUrJPc1TyMVRnEW8jSQeawUQ9qCQCrk9s-iWHMIMbDsBiL1IGdBnoJTGYQSndldslWUhd4jNGQ6ivJQ6lCFQvup1DHPIP71s9zPvcxzyB684URegy1MJpcMM_BgngaCM4fsrl57cmsIWxIcPwBr6pCDDTm0FzDkyeS-cMh-I5jEzvMqYT4PkYYycAhtz4ISxswavKNyUSURh__EooD95hIRIhG8N3DICyPo9dM9IZBY0iHRBgTaC5ACfvNMMbtZUcGDMykCEb_882P3yeP1RHxFtuqvC_0a_OlaHaxA_gNT8Mtt |
| link.rule.ids | 230,315,783,787,888,27936,27937 |
| linkProvider | Geneva Foundation for Medical Education and Research |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Dynamic+Ca2%2B-Dependent+Stimulation+of+Vesicle+Fusion+by+Membrane-Anchored+Synaptotagmin+1&rft.jtitle=Science+%28American+Association+for+the+Advancement+of+Science%29&rft.au=Lee%2C+Han-Ki&rft.au=Yang%2C+Yoosoo&rft.au=Su%2C+Zengliu&rft.au=Hyeon%2C+Changbong&rft.date=2010-05-07&rft.issn=0036-8075&rft.eissn=1095-9203&rft.volume=328&rft.issue=5979&rft.spage=760&rft.epage=763&rft_id=info:doi/10.1126%2Fscience.1187722&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0036-8075&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0036-8075&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0036-8075&client=summon |