Molecular Description and Industrial Potential of Tn6098 Conjugative Transfer Conferring Alpha-Galactoside Metabolism in Lactococcus lactis

• Published in: Applied and Environmental Microbiology Vol. 77; no. 2; pp. 555 - 563
• Main Authors: Machielsen, Ronnie, Siezen, Roland J, van Hijum, Sacha A.F.T, van Hylckama Vlieg, Johan E.T
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.01.2011; American Society for Microbiology (ASM)
• Subjects: Bacteria; Bacteriology; Biological and medical sciences; Carbon; Carbon sources; Chromosomes; Conjugation, Genetic; Dairy products; DNA Transposable Elements; DNA, Bacterial - genetics; Fermentation; Food Industry; Food Microbiology; Fundamental and applied biological sciences. Psychology; Galactosides - metabolism; Gene Transfer, Horizontal; Industrial Microbiology; Lactococcus; Lactococcus lactis - genetics; Lactococcus lactis - metabolism; Lactococcus lactis - physiology; Metabolism; Microbiology; Polymerase Chain Reaction; Recombination, Genetic; Sequence Analysis, DNA; Soy Milk - metabolism; Streptococcaceae; Lactococcus lactis; Lactic acid bacteria; Transfer; Bacteria; Micrococcales; Metabolism

A novel 51-kb conjugative transposon of Lactococcus lactis, designated Tn6098, encoding the capacity to utilize α-galactosides such as raffinose and stachyose, was identified and characterized. Alpha-galactosides are a dominant carbon source in many plant-derived foods. Most dairy lactococcus strain...