Suaveolindole, a new mass-limited antibacterial indolosesquiterpene from Greenwayodendron suaveolens obtained via high-throughput natural products chemistry methods

• Published in: Journal of natural products (Washington, D.C.) Vol. 68; no. 1; pp. 122 - 124
• Main Authors: Yoo, H.D, Cremin, P.A, Zeng, L, Garo, E, Williams, C.T, Lee, C.M, Goering, M.G, O'Neil-Johnson, M, Eldridge, G.R, Hu, J.F
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 01.01.2005; Amer Chemical Soc; American Society of Pharmacognosy
• Subjects: Annonaceae; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; antibacterial properties; Bacillus subtilis; Biological and medical sciences; chemical structure; Chemistry, Medicinal; Enterococcus faecalis - drug effects; Fruit - chemistry; Gabon; General pharmacology; Greenwayodendron suaveolens; indole sesquiterpene; Klebsiella pneumoniae - drug effects; Life Sciences & Biomedicine; Medical sciences; Methicillin Resistance; Microbial Sensitivity Tests; Molecular Structure; Pharmacognosy. Homeopathy. Health food; Pharmacology & Pharmacy; Pharmacology. Drug treatments; phytochemicals; plant extracts; Plant Sciences; Pseudomonas aeruginosa - drug effects; Science & Technology; Sesquiterpenes - chemistry; Sesquiterpenes - isolation & purification; Sesquiterpenes - pharmacology; sesquiterpenoids; spectral analysis; Staphylococcus aureus; Staphylococcus aureus - drug effects; stereochemistry; suaveolindole; Gabon; Africa; LIBRARIES; STEM BARK; ALKALOIDS; Bacillus subtilis; Bacillaceae; Meticillin; Medicinal plant; Bacillales; Carboxylic acid; Dicotyledones; Chemical compound library; Sesquiterpenes; Angiospermae; Bacteria; Micrococcales; Micrococcaceae; Annonaceae; Staphylococcus aureus; Terpenoid; Pharmacognosy; Natural compound; In vitro; Biological activity; Resistance; Plant origin; Isolation; Spermatophyta; Antibacterial agent; Indole derivatives; Structural analysis
• DOI: 10.1021/np040165b
• ISSN: 0163-3864

Utilizing high-throughput isolation, purification, and analysis methods applied to a natural products library, a new mass-limited antibacterial indolosesquiterpene, suaveolindole (1), was obtained from Greenwayodendron suaveolens. The miniaturization of the structure elucidation of 1 was performed primarily using the CapNMR probe. Compound 1 was found to possess significant in vitro antibacterial activity against the Gram-positive bacteria Bacillus subtilis (ATCC 43223), Staphylococcus aureus (ATTC 6538P), and methicillin-resistant Staphylococcus aureus (ATTC 33591), with MIC values of 4, 8, and 8 mug/mL, respectively.