Ratiometric Fluorescent Probe Based on FRET for Imaging Hg²⁺ Ions in Living Cells

Mercury rising: A FRET‐based ratiometric probe (see picture; FRET=fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the parts‐per‐billion scale under physiological conditions. The probe undergoes a clear Hg2+‐induced change in the intensity ratio of the two well‐separated a...

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Published in	Angewandte Chemie International Edition Vol. 47; no. 42; pp. 8025 - 8029
Main Authors	Zhang, Xiaolin, Xiao, Yi, Qian, Xuhong
Format	Journal Article
Language	English
Published	Weinheim Wiley-VCH Verlag 01.01.2008 WILEY-VCH Verlag WILEY‐VCH Verlag Wiley
Subjects	Cell Line, Tumor Cell Survival Chemistry Chemistry, Multidisciplinary Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemistry fluorescent probes FRET Humans Ions - analysis Ions - chemistry mercury Mercury - analysis Mercury - chemistry Mercury - metabolism Molecular Structure Physical Sciences rhodamine Science & Technology sensors DESIGN RECOGNITION mercury SENSOR fluorescent probes RESONANCE-ENERGY-TRANSFER TURN-ON CHEMOSENSOR sensors HG(II) FRET MERCURIC ION rhodamine WATER CHEMODOSIMETER
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Abstract	Mercury rising: A FRET‐based ratiometric probe (see picture; FRET=fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the parts‐per‐billion scale under physiological conditions. The probe undergoes a clear Hg2+‐induced change in the intensity ratio of the two well‐separated and equally intense emission bands of dipyrrometheneboron difluoride and rhodamine. Experiments show that the probe can be used in living cells.
AbstractList	Mercury rising: A FRET‐based ratiometric probe (see picture; FRET=fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the parts‐per‐billion scale under physiological conditions. The probe undergoes a clear Hg2+‐induced change in the intensity ratio of the two well‐separated and equally intense emission bands of dipyrrometheneboron difluoride and rhodamine. Experiments show that the probe can be used in living cells.
Author	Qian, Xuhong Zhang, Xiaolin Xiao, Yi
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Cites_doi	10.1021/ja068879r 10.1021/ol070109c 10.1021/ja037604y 10.1021/tx0501536 10.1002/anie.200702854 10.1021/ja0557987 10.1021/ja711480f 10.1021/ja063784a 10.1021/ol062867t 10.1021/ja058007+ 10.1021/bi035480w 10.1021/ja063308k 10.1021/ja066144g 10.1021/jo052642g 10.1021/ol0529086 10.1021/cr078381n 10.1021/ol702869w 10.1039/b511319a 10.1021/jo071226o 10.1002/anie.200454172 10.1021/ja054855t 10.1021/ja0290779 10.1002/anie.200701785 10.1021/ol7020143 10.1021/ja068342d 10.1002/anie.200250788 10.1002/anie.200702006 10.1021/ol0610340 10.1039/b617972j 10.1021/ol0708931 10.1002/anie.200503873 10.1021/ja0643319 10.1002/ANGE.200702006 10.1021/ol702558p 10.1021/ja060399c 10.1021/ja065114a
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Keywords	DESIGN RECOGNITION mercury SENSOR fluorescent probes RESONANCE-ENERGY-TRANSFER TURN-ON CHEMOSENSOR sensors HG(II) FRET MERCURIC ION rhodamine WATER CHEMODOSIMETER
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Notes	http://dx.doi.org/10.1002/anie.200803246 Program for Changjiang Scholars and Innovative Research Team in University - No. IRT0711 istex:F0A4CFE6D2D4B91E2E6628D3E6AC9C01D9F74E8D NSF of China - No. 20406004; No. 20572012; No. 20536010 ArticleID:ANIE200803246 We thank the NSF of China (Nos. 20406004, 20572012, 20536010) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT0711) for financial support. We also thank Dr. Liji Jin and Xueliang Niu (Dalian University of Technology) for help with cell culture experiments. ark:/67375/WNG-XXRT3S5W-3 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
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Snippet	Mercury rising: A FRET‐based ratiometric probe (see picture; FRET=fluorescence resonance energy transfer) can selectively detect Hg2+ ions on the...
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SubjectTerms	Cell Line, Tumor Cell Survival Chemistry Chemistry, Multidisciplinary Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemistry fluorescent probes FRET Humans Ions - analysis Ions - chemistry mercury Mercury - analysis Mercury - chemistry Mercury - metabolism Molecular Structure Physical Sciences rhodamine Science & Technology sensors
Title	Ratiometric Fluorescent Probe Based on FRET for Imaging Hg²⁺ Ions in Living Cells
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