Tomato exo-(1 leads to 4)-beta-D-galactanase: isolation, changes during ripening in normal and mutant tomato fruit, and characterization of a related cDNA clone

• Published in: Plant physiology (Bethesda) Vol. 108; no. 3; pp. 1099 - 1107
• Main Authors: Carey, A.T. (Horticulture Research International, Wellsbourne, Warwick, UK.), Holt, K, Picard, S, Wilde, R, Tucker, G.A, Bird, C.R, Schuch, W, Seymour, G.B
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.07.1995
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADN; Amino Acid Sequence; Amino acids; beta-Galactosidase - genetics; beta-Galactosidase - isolation & purification; beta-Galactosidase - metabolism; Biochemistry and Enzymology; Biological and medical sciences; Carbohydrate Sequence; Catalysis; Cell walls; Chromatography, Gel; Chromatography, Ion Exchange; CLONE; CLONES; Cloning, Molecular; Complementary DNA; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; Electrophoresis, Polyacrylamide Gel; Enzymes; ETAPAS DE DESARROLLO; FRUITS; FRUTAS; Fundamental and applied biological sciences. Psychology; Galactans; GALACTOSIDASAS; GALACTOSIDASE; Gels; GENETICA; GENETIQUE; Glycoside Hydrolases; Isoenzymes - genetics; Isoenzymes - isolation & purification; Isoenzymes - metabolism; LYCOPERSICON ESCULENTUM; Lycopersicon esculentum - enzymology; MADURAMIENTO; MEDIO DE CULTIVO; Metabolism; MILIEU DE CULTURE; Molecular Sequence Data; MURISSAGE; MUTANT; MUTANTES; Plant physiology and development; Plants; PROPIEDADES FISICO-QUIMICAS; PROPRIETE PHYSICOCHIMIQUE; PROTEINAS; PROTEINE; Proteins; PURIFICACION; PURIFICATION; REACCIONES QUIMICAS; REACTION CHIMIQUE; Ripening; Sequence Homology, Amino Acid; STADE DE DEVELOPPEMENT; Substrate Specificity; VARIACION GENETICA; VARIATION GENETIQUE; Fruit; Enzyme; Homology; Characterization; Ripening; Enzymatic activity; Complementary DNA; Dicotyledones; Substrate specificity; Angiospermae; Lycopersicon esculentum; N terminal-Sequence; Isolation; Spermatophyta; Solanaceae

An exo-(1 to 4)-beta-D-galactanase was isolated from ripe tomato fruit (Lycopersicon esculentum Mill. cv Ailsa Craig and cv Better Boy) using anion-exchange, gel filtration, and cation-exchange chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the most active fraction reve...